Study On New Lateral Flow Strip Methods Based On Recognition Of Kanamycin With Aptamer

Lateral flow strip has the advantages of simple operation,portability,rapid detection,and can be used in real-time detection and clinical detection,so it has been widely used.The surface of gold nanomaterial is easy to modify and has special optical properties and certain catalytic ability.Using the properties of gold nanomaterial,it was applied to the lateral flow strip as a signal molecule,and a method for the detection of kanamycin was established.1.In this paper,a new type of colloidal gold lateral flow strip was fabricated based on aptamer recognition of kanamycin molecule and non-thiolated DNA modified colloidal gold nanoprobe.A highly sensitive detection of kanamycin was achieved through the optimization of components on the strip and particularly the aptamer strand.The results demonstrated that25 ng/m L kanamycin could be discriminated by the naked eyes.The quantitative determination of kanamycin could be achieved in the range of 5.0 ng/m L-125 ng/m L with a detection limit of 1.5 ng/m L by colloidal gold reader.The test strip was applied to the determination of kanamycin in honey and the recoveries from 95.1%to105.2%were obtained.The strip has the characteristics of high sensitivity,simple structure,good selectivity,simple operation and high repeatability.2.A universal lateral flow strip was designed to detect another kind of biomolecule without changing NC membrane.Based on the competition between kanamycin and the complementary sequence of aptamer,kanamycin can be detected quickly and sensitively.By optimizing the probe sequence,the high sensitive detection of kanamycin was realized.The naked eye resolution concentration of kanamycin was 7.5 ng/m L,and the quantitative determination of kanamycin in the concentration range of 0.5 ng/m L-250 ng/m L could be achieved through the quantitative analysis of colloid gold strip,with the minimum detection limit of 0.3 ng/m L.In addition,the method was also used for the determination of kanamycin in samples,and the recovery rate was 94%-103.8%,which indicated that the method has a certain application potential.3.A novel signal amplified lateral flow strip of nanometer mimicking enzyme was constructed to realize the rapid and sensitive detection of kanamycin.The continuous guanine-cytosine DNA strand was modified to Au NPs.Continuous guanine-cytosine DNA strands were modified into AUNPs to bind Cu²⁺in a unique coordination pattern.Cu²⁺had a strong peroxidase-like activity,thus increasing the peroxidase activity of gold nanoparticles.DAB was used as an oxidation substrate to amplify the signal of the paper strip.The minimum concentration of kanamycin identified by naked eyes was 1 ng/m L,and the quantitative determination of kanamycin in the concentration range of 0.1 ng/m L-500 ng/m L could be achieved by colloidal gold quantitative analysis,with the minimum detection limit of 0.12 ng/m L.The recovery was 95.3%-105.5% in honey samples.