Application Of Porous Materials In The Enrichment Detection Of Indole-3-Methanol And Sulforaphane

Vegetables are rich in bioactive substances,and the current traditional extraction methods have disadvantages,such as the use of excessive organic solvents and adverse effects on the environment and human body.Functionalized porous materials are porous materials with one or more of the functions of adsorption,catalysis,magnetism,electrical conductivity or light absorption in a mesh structure of interpenetrating or closed pore channels,of which both metal organic framework materials(MOF)and covalent organic framework materials(COF)have been substantially applied in the fields of pesticide adsorption,energy storage and catalysis.In this study,a qualitative and quantitative method based on high performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)was developed for the quantification of indole-3-methanol and sulforaphane.A magnetic composite MOF was prepared for the adsorption and enrichment of indole-3-methanol from vegetable enzymatic digest,which achieved the purpose of avoiding the use of organic solvents.A method for the adsorption-elution and detection of sulforaphane was developed by preparing a complex of magnetic MOF and COF,which supports the enrichment and purification of sulforaphane for use in real samples.1.A successful method for the determination of indole-3-methanol and sulforaphane based on HPLC-MS/MS.The indole-3-methanol assay showed good linearity in the range of 5-1000 ng/m L with R²=0.999;The sulforaphane assay showed good linearity in the range of 5-300 ng/m L with R²=0.991.The limit of detection(LOD)of indole-3-methanol was 1.02 ng/m L and the limit of quantification(LOQ)was 5ng/m L;the LOD of sulforaphane was 0.5 ng/m L and the LOQ was 2.5 ng/m L.The established method has less interference and high sensitivity,accuracy and precision.Also aqueous solutions of indole-3-methanol and sulforaphane are stable at room temperature.The final application was for the qualitative and quantitative analysis of indole-3-methanol and sulforaphane in broccoli and green kale with RSD ranging from0.57?2.57%.2.The composite Fe₃O₄-MWCNT-MIL-88A@Au was prepared by co-precipitation and surface polymerization for the rapid and efficient enrichment of indole-3-methanol.The Fe₃O₄-MWCNT-MIL-88A@Au composites were characterized by various characterization tools to determine the structure and properties of the MOFs.The calculated BET specific surface area and pore size were 98.3708 m2/g and 20.5348 nm,respectively.The adsorption conditions of Fe₃O₄-MWCNT-MIL-88A@Au on indole-3-methanol were optimized,and the experimental results showed that Fe₃O₄-MWCNT-MIL-88A@Au had excellent adsorption on indole-3-methanol.A method for the enrichment and purification of indole-3-methanol in orange juice,apple juice and the enzymatic digest of green kale and broccoli was constructed under optimal experimental conditions in combination with HPLC-MS/MS and quantified qualitatively.3.MOF and COF hybrid composites Fe₃O₄-ZIF-8@COF were prepared by in situ polymerization,and were enrichment with sulforaphane within complex matrices.The structure of Fe₃O₄-ZIF-8@COF was determined by various characterization means,and the calculated BET specific surface area and pore volume were 54.7793 m²/g and0.372220 cm³/g,respectively.The optimal adsorption-desorption conditions were obtained through optimization of adsorption and desorption experiments,and the method for the enrichment of sulforaphane in fruit and vegetable samples by HPLC-MS/MS was successfully constructed.The recoveries were 76.7-78.2%with RSDs of0.30-1.24%for apple juice and 72.6-75.8%with RSDs of 0.29-0.94%for orange juice when added at three levels of 10,50 and 100?g/L.