| In order to realize the high-value utilization of peanut red coat,this study took proanthocyanidins in peanut skins(PSPC)as the research object.Through extraction,separation and purification,the proanthocyanidins with high purity were obtained.Then,the composition was analyzed,and the related functional evaluation was carried out at last.The results are as follows:(1)Using ethanol solution as the extraction solvent,the extraction process of procyanidins was optimized by single factor and orthogonal experiments.The results showed that the main factors affecting the extraction efficiency of procyanidins were as follows: ultrasonication time > ethanol volume fraction >solid-liquid ratio > ultrasonication temperature;The optimal extraction conditions were as follows:ultrasonic time 15 min,ethanol volume fraction 60%,solid-liquid ratio(g/m L)1:45,ultrasonic temperature35 ?,water bath temperature 50 ?,water bath time 50 min.Under these conditions,the average maximum extraction yield of proanthocyanidins was 9.07%,which was higher than that of ultrasonic extraction or water bath extraction alone.(2)By static adsorption and desorption experiment to select eight types of macroporous resin,the best types was D4020,use this type of resin for dynamic test,the sample flow rate,sample concentration and the concentration of eluent,elution velocity on several aspects,it is concluded that the best purification scheme for the sample flow rate of 1 m L/min,the sample concentration of 1.5 mg/m L,eluent is 40%ethanol,the elution flow rate of 1 m L/min,finally the purified material purity can reach 97.35±1.58%.UPLC-Q-TOF /MS analysis showed that the peak time of the purified compounds was relatively concentrated within 4-7 min,and there were at least 5 haploids and 10 proanthocyanidin oligomers.Haploid contain catechin,table gallic catechin gallic acid ester,the original catechin and epicatechin derivatives,such as A type of oligomers 4 kinds of oligomers,B type six oligomers,has identified the dimers by epicatechin monomer by type A key connections,or by the epicatechin gallic acid ester and catechins by type B connection key links,trimer epicatechin monomer by type B connection key links,or by the epicatechin and catechins by type B connection key links.(3)By studying the biological activity of PSPC,the results showed that the proanthocyanidins had strong scavenging effect on DPPH free radical and ABTS free radical,both of which were positively correlated with the mass concentration of proanthocyanidins,and the scavenging ability of proanthocyanidins on DPPH and ABTS free radical was obviously stronger than that of ascorbic acid.The acid value,POV and TBA values of pork fat impregnated with PSPC and VC were significantly lower than those of blank group.In the determination of acid value,the inhibition effect of 0.1% PSPC was the best,and the inhibition effect of 0.1% VC solution was stronger than that of 0.05%,0.15% and 0.2% PSPC.In terms of POV measurement,0.2% of peanut erychlamydia anthocyanin had the best inhibition effect,while0.1% of VC had roughly similar inhibition effect to 0.15% of PSPC.In the measurement of TBA value,0.2% of peanut erychlamydia anthocyanin had the best inhibitory effect,and 0.1% of VC had the inhibitory effect between 0.15% and 0.2% of PSPC.Both ?-Glu and ?-Amy were inhibited by PSPC.The inhibition effect of acarbose on ?-Glu was better than that of proanthocyanidin,while the inhibition effect on ?-Amy was better than that of proanthocyanidin.In the in vitro digestion test,the digestibility of PSPC was low in the gastric environment,with the digestibility of only 14.2% after 2.5 hours of gastric environment reaction,and 54.1% after 2.5 hours of intestinal environment reaction.The results showed that the digestion effect of PSPC was not obvious in the stomach environment,but the digestion effect was better in the intestinal environment,that is,the main digestive and absorption organ of PSPC in the body was the intestinal tract. |
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