Preparation And Photodynamic Activity Of MOF Nanomaterials

Cancer is one of the major diseases threatening human health,and is also a major public health problem worldwide.As is known to all,surgical treatment,radiotherapy and chemotherapy are the clinical routine treatments for cancer.However,as a result of poor therapeutic effect,high risk of recurrence and unbearable side effects,the routine treatment often cannot meet the needs of the clinical practice.Photodynamic therapy（PDT）is a kind of cancer treatment developed in recent decades.It depends on the non-toxic photosensitizer and oxygen to trigger reactive oxygen species（ROS）to kill tumor cells.But during the process of photodynamic therapy,the poor water-soluble of photosensitizer,easy aggregation in a physiological environment,the skin phototoxicity have affected the photodynamic effect.Meanwhile,hypoxia tumor microenvironment seriously limits the generation of ROS,greatly influencing the effect of PDT.Unique properties of nanoparticles（NPs）can improve the traditional PDT effect and overcome some shortages of PDT,therefore nanomaterials have great potential in tumor PDT.Among various nanomaterials,the metal-organic skeleton（MOF）nanomaterials have gained much attention because of its varied structure,easy modification property,aboundunt permanent porosity and good biocompatibility.To overcome the poor water-soluble,easy aggregation in a physiological environment of photosensitizer,this paper designed a MOF-PPa@PAH nanometer platform.The MOF was prepared through solvent hot method,and then MOF-PPa@PAH was prepared by physical mixing.Under 660 nm laser irradiation,the nanometerials can kill cancer cells by ROS.In vitro experiments of MOF-PPa@PAH against HepG-2 cells showed excellent cell toxicity,and ignorable dark toxicity.We also studied the PDT machnism of the materials,which showed that type I and type II photodynamic reaction occurred simultaneously,and type II reaction played a main role.Moreover,the MOF-PPa@PAH could be cellular uptaken into the tumor cells quicker than that of free PPa,and the photosensitizer PPa could be released rapidly in the cells.On the other hand,we also tried to load a layer of MnO₂ shell on the surface of MOF-PPa to alleviate the tumor microenvironment hypoxia,considering that MnO₂ could react with H₂O₂ under light in the tumor microenvironment to generate ROS oxygen reaction under light,subsequently improving the PDT effect.