Design,Preparation And Properties Of Non-spherical ?-Fe₂O₃ Fluorescent Nanoparticles-based Gene Vector

With the in-depth research of gene therapy in recent years,people are more and more aware that choosing a suitable vector to safely and efficiently deliver the therapeutic gene to the tumor site and achieve expression is the key to the success of gene therapy.Among them,the inorganic/organic composite nanoparticles composed of cationic polymers and inorganic nanomaterials have stable structure,easy preparation and modification,good biocompatibility,non-immunogenicity,and other excellent physical and chemical properties,which makes it a great space for development as a carrier Has attracted widespread attention and has become a hotspot of current research.In this paper,biocompatible inorganic ?-Fe₂O₃nanoparticles are used as a model system,by combining the hydrothermal method with the sol-gel method,a tetraphenylenevinylsiloxanederivative(AIEgen-Si(OCH₃)₃)with aggregation-induced luminescence characteristics was introduced into a newly formed thin Si O₂layer on the surface of the nanoparticles,and synthesized Two kinds of non-spherical(ie ellipsoidal and spindle-shaped)fluorescent ?-Fe₂O₃nanoparticles were developed.Then,they were modified with polyethyleneimine(PEI)and triethylamine to obtain four gene vectors,and their properties were characterized.The main contents are as follows:1?Preparation and characterization of ellipsoidal fluorescent ?-Fe₂O₃nanoparticle gene carrier.Ellipsoidal ?-Fe₂O₃nanoparticles(HNP)were prepared by adjusting the p H value of the aqueous solution using the hydrothermal method;the nanoparticles were mixed with tetraethyl orthosilicate(TEOS)and tetraphenylenevinylsiloxane derivative(AIEgen-Si(OCH₃)₃)The two silanization reagents are mixed and subjected to a sol-gel reaction to prepare composite fluorescent ?-Fe₂O₃nanoparticles(FHNP)with luminous properties;then PEI and triethylamine are mixed with iodine on the surface of the fluorescent nanoparticles The propyl group was subjected to a nucleophilic substitution reaction to obtain two ellipsoidal fluorescent ?-Fe₂O₃nanoparticle gene vectors(FHNP-PEI and FHNP-NEt₃).Use transmission electron microscope(TEM),X-ray photoelectron spectroscopy(XPS),thermogravimetric analysis(TGA),Fourier transform infrared spectroscopy(FTIR),dynamic light scattering(DLS),ultraviolet-visible spectrum,fluorescence spectrum,etc.A biophysical detection method characterizes its properties.The results showed that the prepared fluorescent ?-Fe₂O₃nanoparticle gene carrier exhibited a uniform ellipsoid shape and a regular morphology.The average grain diameter was 232 nm and 223.1 nm,and the zeta potential was+33.9 m V and+6.13 m V,respectively.The agarose gel electrophoresis blocking experiment proved that the nanoparticle gene vectors have a good binding ability to calf thymus DNA.Cytotoxicity experiments proved that the two kinds of nanoparticle gene vectors have low toxicity to Hela cells,and have good biocompatibility.They are potential nanogene vectors.2?Preparation and properties of spindle-shaped fluorescent ?-Fe₂O₃nanoparticle gene carrier.Similarly,we prepared two spindle-shaped fluorescent ?-Fe₂O₃nanoparticle gene vectors(FSNP-PEI and FSNP-NEt₃).The analysis results showed that the prepared fluorescent ?-Fe₂O₃nanoparticle gene carrier exhibited a uniform spindle shape and regular morphology.The average grain diameter was 282.1 nm and280.2 nm,and the zeta potential was+23.9 m V and+9.34 m V,respectively.It has good binding ability to calf thymus DNA,and has good biocompatibility.