Separation And Analysis Of Organic Small Molecules And Phosphorylated Proteins Based On Functional Composite Nanomaterials

Due to its unique structure and properties,functional nanocomposites have become a research hotspot in many field.In this paper,functional composite nanomaterials are successfully prepared and applied to analysis organic small molecule pollutants and proteomics.A series of new technologies and strategies have been developed and applied in biosamples.The main research contents and results are summarized as follows:（1）A series of functional nanocomposites have been developed and successfully introduced into the detection of phthalic acid esters（PAEs）in practical samples.Firstly,a C₁₈ modified magnetic graphene / polydopamine hybrid material mag@PDA@C₁₈ was designed,and 6 PAEs in tap water and bottled water were successfully detected.Inspired by relevant reports,functional ionic liquids were modified into magnetic substrates and ATP was introduced into materials.The Fe₃O₄@PDA@ATP@IL magnetic nanocomposites were successfully enriched 6 PAEs from human serum.As a new star in the field of materials science,the metal organic frameworks materials（MOF）have attracted extensive attention in various fields.Inspired by this,we designed two kinds of MOF composite materials mag@PDA@ZIF-8 and mag@ZIF-8.Among them,mag@PDA@ZIF-8 has better hydrophilicity,magG@ZIF-8 is synthesized by using SiO₂ as the protective shell of graphene and as a sacrificial template.Due to the combination of MOF structure,these two materials have higher specific surface area,regular channels and can enrich phthalate esters（PAEs）through π-π interaction and hydrophobic interaction.Covalent organic frameworks（COF）is a new type of crystalline porous body,which has been widely used since its discovery.COFs has excellent structural regularity,high specific surface area,low density and adjustable pore structure.Due to these permits,we have designed Fe₃O₄@PDA@Tb Bd and mag@PDA@Tb Bd,this is the first time to apply the COFs composite to PAEs detection.This work provides a new idea for the separation and analysis of organic small molecule pollutants.（2）Two functional nanocomposites have been developed and successfully introduced into the study of phosphorylation proteomics.Firstly,a kind of IMAC composite nanomaterial Fe₃O₄@PDA@ATP-Ti⁴⁺ was synthesized.The synthesis procedure of this material is simple and the amount of fixed Ti⁴⁺ is large.It was used to study the phosphopeptides in the standard phosphatase hydrolysate,human saliva and human serum.The results show this material has a good enrichment effect on the phosphopeptides.In addition,a new kind of MOAC composite Fe₃O₄@PDA@TiO₂@Cu₃（BTC）₂ can be synthesized in one step by using ionic liquid as solvent.The method of is simple and environmentally friendly.At the same time,it has larger specific surface area and hydrophility.This material shows potential application prospects in the concentration of phosphopeptides in human saliva and serum.