| The core innovation of this study is to simulate the liquid-crystal-like structure of extracellular matrix（ECM）.The binary complex liquid crystal systems of GO/HA and GO/SA were prepared by using GO（GO）liquid crystal（LCs）as the liquid crystal state element and HA/alginate（SA）as the matrix.The results show that the critical concentration of GO liquid crystal can be significantly reduced（about 10 times）in the liquid crystal system constructed with hyaluronic acid and alginate,and such liquid crystal system has a long-range orientation structure（The existence of multiple liquid crystal domains in pure GO liquid crystal is short-range orientation）.The core technology of this study was using freeze drying and Sr2+,Mn2+,Ca2+crosslinking modification technology to build the system of dual LCD 3D scaffolds,not only to keep the long-term orientation of liquid crystal state and porous mutual communication structure,also markedly enhance the mechanical strength of scaffolds（compression strength by about 5 times）,even if GO content is very low,it still has excellent mechanical properties.The results of in vivo and in vitro experiments in this study showed that both Ca2+or Sr2+cross-linked liquid crystal scaffolds and self-assembled liquid crystal fiber membranes were conducive to the adhesion,spread,proliferation and osteogenic differentiation of human bone marrow mesenchymal fine tissue（h BMSCs）,and had obvious skull defect repair activity.In this paper,a new method for the preparation of scaffolds with excellent structure and performance is developed,which has a good application prospect.Liquid crystal cell culture medium was prepared based on GO/HA liquid crystal system to simulate the growth environment of cells in the liquid crystal state in vivo.The proliferation experiment of h BMSCs cells showed that the medium had good biocompatibility and was conducive to cell growth,which opened up a new system for cell culture. |
PDF Full Text Request