| With the developing large-scale,intensive and modernized dairy cow breeding industry in NingXia.It is pursuing green,healthy and sustainable development.Dairy cows have a large amount of farming and a lot of manure.After solid-liquid separation,the solid microbial fermentation is used for agricultural purposes,and total consumption spends much time;the liquid enters the anaerobic tank,which is not completely anaerobic,and the biogas is discharged,power generation,heating,as well as using by vehicles after purification;The COD value of the fermented biogas slurry is very high and cannot be directly used in farmland.It further enters the oxidation pond,evaporates naturally,pollutes the environment,wastes resources.The traditional solid manure microbial aerobic fermentation and the fermentation of commercial organic fertilizers need to meet the NY525-2012 standard,but the organic matter content is not enough.A large amount of weathered coal or furfural residue is added.These two kinds of exogenous substances which lacks of nutritional value are added up to 50%around N,P,and K nutrients are not enough,adding inorganic fertilizers to maintain nutrient activity,while more nitrogen loss.The study aimed at the slow rate of solid manure fertilizer in farms,low efficiency,nutrient loss,low anaerobic fermentation gas production and higher COD value of the feed liquid,and the introduction of Taiwan Ditiantai Agricultural Biotechnology Co.Ltd.closed rotating heating.The rapid enzymatic fermentation of agricultural waste resources to make organic fertilizer technology,research on the co-enzymatic rapid fermentation process of solid manure from different dairy cattles and corn stalks and the physical,chemical and biological properties and fertilizer efficiency of the materials after enzymatic hydrolysis;Incompletely,anaerobic fermentation of biogas slurry and cow manure mixed with corn stalks pretreated with different acetic acid concentrations in high-efficiency anaerobic fermentation process,to explore the effectiveness and feasibility of enzymatic hydrolysis technology in the use of fertilizers,and the gas production of secondary biogas slurry utilization Whether the biogas slurry can be returned to the field.The main researches are as follows:1.Enzymatic hydrolysis technology and product quality:the ratio of cow dung and corn stalks is adjusted,the C/N ratio is adjusted to 25/1,and the special enzyme for cow dung(neutral protease)is evenly sprinkled in the proportion of 2%.Heating at 80℃ and rotating oxygen,after only 3 hours of enzymatic hydrolysis,the product has no foul smell,powdery,no impurities,and brown in color:the water content of the material after enzymatic hydrolysis is reduced by 10%,but it is higher than the NY525-2012 organic fertilizer standard.The material needs further airing or drying treatment;the organic matter content is gradually decreasing,in line with the NY525-2012 organic fertilizer standard,available phosphorus and potassium,total salt and humic acid are on the rise,total nitrogen,phosphorus and total potassium and nutrients have reached more than 5%,in line with the NY525-2012 organic fertilizer standard;the lignin content has not changed a lot,the cellulose and hemicellulose content has been significantly reduced,and the rate of dry-based fecal roundworms can reach 95%,biogas residue and raw cow manure after enzymatic hydrolysis,the mortality of roundworms is close to 95%,and the germination index of Chinese cabbage seeds reached 80%,reaching the standard of organic fertilizer degradation;the number of bacteria,fungi,and actinomycetes decreased,and the content of As,Pb,Cd,and Cr did not exceed NY The limit value of 525-2012 meets the limit standard of organic fertilizer.The content of Zn(135.51,132.42,131.60 mg·kg-1)and the content of Cu(29.01,17.22,22.18 mg·kg-1)are also listed in GB 4284-2018.The limited scope of "Agricultural Sludge Pollutants Control Standard".2.Efficient anaerobic fermentation process:Corn stalks are pretreated with different concentrations of acetic acid and fermented under medium temperature conditions,with normal temperature anaerobic fermentation as a control.The PH value of different concentrations of acetic acid decreased rapidly in the early stage of fermentation,and then increased rapidly and gradually became a stable state;volatile fatty acids(VFA)generally showed a "Λ" type change,and the maximum value of acetic acid pretreatment appeared on the 10d.Hydrogen salinity alkalinity(TIC)showes a rapidly decrease and then gradually increased;the range of reducing sugar content is generally not large,but generally showed a decreasing trend.The reducing sugar content of each treatment was 7.46~15.81 mg·mL-1;the control and the cellulase activity of normal temperature fermentation treatment is lower than that of acetic acid pretreatment;the ammonia nitrogen concentration showes a gradually increasing trend,and the ammonia nitrogen concentration of each treatment is maintainning at 133.74~419.25 mg·L-1;There are obvious differences in the quality of Fe2+ in the oxygen digestion experimental system;the content of BOD5 and CODcr pretreated with acetic acid is significantly reduced,but neither of them reaches the limit value of the "Emission Standard of Pollutants from Livestock and Poultry Breeding".In the entirely anaerobic digestion stage,the gas production of acetic acid pretreatment is significantly higher than that of room temperature fermentation.Among them,the gas production of 6%acetic acid concentration pretreatment was the highest,which was 10745 mL,which was 9910 mL more than room temperature fermentation(835 mL),laying the foundation for the fertilizer-based agricultural use of biogas slurry after full anaerobic fermentation.3.Fertilizer efficiency of the enzymatic hydrolysis products:The fermented product after enzymatic hydrolysis is compared with no fertilization,conventional fertilization,commercial organic fertilizer for planting peppers.The results show that cowss talks can completely replace weathered coal,furfural residue and cow dung.Organic fertilizer and the application of enzymatic hydrolysis products can significantly promote the growth and development of peppers and root morphology,improve the fertility of the subsoil,increase pepper resistance and yield-related indicators,iin order to mprove pepper quality,increasing soil enzyme activities,and promoting the formation of microflora.The total amount of Cu and Zn in the soil increased,but no pollution occurred.After a comprehensive analysis of the components of the enzymatic hydrolysis products and the growth and development indicators of peppers.It is concluded that dung residue enzymatic hydrolysis products are better than the biogas residue and dry-based dung enzymatic hydrolysis products,but they are both beneficial to the growth and physiology of peppers and increase the yield of peppers per plant.To I mprove rhizosphere soil enzyme activity,provide a good living and breeding place for microorganisms,and enrich the number of soil microorganisms. |