Study On The Regulation Of Electrochemiluminescence Of Polyadopamine Nanoparticles And Bioanalysis Application

Electrochemiluminescence(ECL)is generated by the excited state originate from the chemical reaction of electrically induced free radicals.Compared with traditional ECL emitters,organic nano-ECL emitters have many advantages such as their superior ECL efficiency and flexible ECL regulation.Polydopamine nanoparticles(PDA NPs)are a type of organic nanoparticles formed by polymerization of dopamine.They have good biocompatibility and significant hydrophilicity,which overcomes the intrinsical limitations of hydrophobic organic ECL nanoparticles.However,the internal structure of PDA NPs is complex and disordered,which leads to problems such as low luminous efficiency,poor optical performance,and so on.Therefore,we have regulated the ECL properties of PDA NPs for the sensing application.In the first chapter of the paper,we reviewed the existing organic ECL luminescent materials,including organic molecular emitters,organic nano-emitters,aggregation induced ECL emitters and polymer emitters.We summarized the applications of organic nano-luminescent materials in the ECL field in recent years.And we briefly described the research significance and main content of this paper.In the second chapter of the paper,we used L-cysteine to reduce the confusion of the internal structure to improve the ECL property of PDA NPs effectively.The surface of the obtained nanoparticles was further modified by polyethyleneimine(PEI).The prepared PDAcp NPs had 10 times ECL intensity higher than PDA NPs.An inner filter effect-based ECL aptasensing and image analysis were accomplished for the detection of adenosine triphosphate(ATP).In the third chapter of the paper,phenylboronic acid(PBA)was used to increase the ECL signal of PDA NPs and adjust the ECL emission band gap.PDA NPs with PBA were synthesized as a novel kind of organic nano-luminophores.The PDA-PBA NPs had high ECL efficiency and regulable emitting wavelength(552?610 nm).Mo S₂nanosheet metal-aerogel played the important role in the improvement of ECL sensing performance.On the one hand,Mo S₂ nanosheet metal-aerogel can load a large number of PDA-PBA NPs.The accommodation of PDA-PBA NPs in the Mo S₂nanosheet metal-aerogel kept the dispersed state,which prevented NPs aggregation and ECL quenching effect.On the other hand,the excellent conductivity of Mo S₂nanosheet metal-aerogel significantly facilitated the electron transfer.Electron injection with Mo S₂ nanosheet metal-aerogel can effectively reduce the damage of PDA-PBA NPs by excessive hot electrons.Finally,a target-catalyzed hairpin assembly sensing mode was designed to visually detect Mi RNA-126.The Mo S₂nanosheet metal-aerogel-based sensing system provided a new pathway in the development of ECL analysis research.