Preparation Of Laccase-loaded Magnetic Nanoflowers And Its Application In Bisphenol A Degradation

Bisphenol A(BPA)is a widely used chemical raw material which is easily accumulated and difficult to remove from the environment.It can lead to reproductive system toxicity and has a serious impact on organisms.The presence of BPA in aqueous solution seriously threatens the health of human beings and other organisms.Until now,there is no efficient and economical treatment for the removal of BPA from water bodies.To develop highly efficient,easy separation,recyclable and reusable materials for BPA degradation in water,laccase-loaded magnetic nanoflowers(MNFs)were successfully prepared.MNFs were characterized by scanning electron microscopy,transmission electron microscopy,Fourier transform infrared spectroscopy,fluorescence microscopy,XRD and vibrating sample magnetometer.We also investigated the degradability and reusability of MNFs for BPA solution.On the basis of this,the preparation method of MNFs was also improved.Characterization results showed that MNFs were spherical,porous and hierarchical structure with diameter of 5 ～ 18 μm.The loading capacity of MNPs was about 100 mg/g.MNFs exhibited excellent catalytic activity on BPA degradation under room temperature.Under optimized conditions(10 μg/m LBPA,0.6 mg/mL MNFs,pH 5.5,0.3 mM ABTS),MNFs reached over 99% BPA degradation for only 5 min.In addition,the MNFs could be recycled conveniently by an external magnet.When it was recycled and reused 5 cycles,only 5% decrease in degradation efficiency of BPA was observed.However,the MNFs obtained by the meliorated preparation method still maintained over 99% degradation of BPA after being recycled and reused for 13 cycles.These results suggest that MNFs possess great efficiency and reusability in the treatment of aqueous solution containing BPA,and is a novel promising laccase immobilization material with efficiency,economy and recoverability.