| Chemometrics provides a new solution to the problem of extracting useful information of specific components from unknown samples with complex components and severe interference and performing accurate and effective qualitative and quantitative analysis on them.Based on its unique“second-order advantage”,the multiway calibration method based on the multi-linear component model replaces or enhances traditional physical or chemical separation methods with fast and green“mathematical separation”,greatly reducing the complicated pre-processing steps,and making the quantitative analysis and detection process more time-saving and labor-saving.The current research mainly focuses on the application of the basic theory of multi-way calibration method combined with high-order analytical instruments in complex quantitative analysis.The main cotent of this thesis includes the following three parts:In Chapter 2:Ofloxacin,lomefloxacin and ciprofloxacin are chemically synthesized antibacterial drugs,which belong to fluoroquinolones(FQs).FQs has broad antibacterial spectrum,low toxicity,as well as good pharmacokinetic characteristics,and is one of the most commonly used drugs in the veterinary clinic.With the development of society and the improvement of people's living standards,the consumption of animal-derived foods is increasing day by day.Therefore,the detection of veterinary drug residues in animal-derived foods through effective technical means is of great significance for food safety.Due to the similar structure of these three substances,their fluorescence spectrum overlaps severely.Without separation,it cannot be measured by conventional methods.In this chapter,to quickly and quantitatively analyze the ofloxacin,lomefloxacin and ciprofloxacin,the second-order calibration method based on the alternating normalization-weighted error(ANWE)algorithm in chemometrics is combined with excitation-emission matrix fluorescence spectroscopy,and the chemical separation process is replaced by“mathematical separation”in part or all.The average recoveries of the three fluoroquinolones in different matrixes were 82.6%-110.5%,the relative standard deviations were less than 7.4%,and the detection limit ranged from 0.18 to 2.41 ng m L-1.For further evaluation,parameters such as sensitivity and selectivity,as well as RSDs values for intra-day precision(?10.6%)and inter-day precision(?9.4%)were calculated.Compared with traditional methods,the sample preparation steps of this method are simple.And in the presence of overlapping peaks and unknown interference,satisfactory results can still be obtained.The method has good linearity(r>0.99),and can be used as an effective strategy for accurate and rapid quantitative analysis of fluoroquinolone drugs such as ofloxacin,lomefloxacin and ciprofloxacin in different anima-derived matrixes.In Chapter 3:Antipsychotic drugs have mild extrapyramidal adverse reactions when they are used.In severe case,serious dangers may occur and life would be threatened.At present,our country often tends to use a combination of multiple drugs in the clinical treatment of schizophrenia.Therefore,in order to guide the safe use of such drugs,real-time monitoring of blood drug concentration provides important clinical guidance significance for the treatment of acute and chronic psychiatric disorders such as schizophrenia and depression.This chapter is based on the self-weighted alternating trilinear decomposition(SWATLD)algorithm,which uses EEM fluorescence data combined with a second-order calibration method to quickly detect sulpiride(SPD)and amisulpride(ASPD)in human serum.Even if the spectra of the target analytes,the background and other unknown interferences overlap significantly,the strategy can still accurately analyze the EEMs data and obtain satisfactory and reliable quantitative results.In human serum samples,the average recoveries of SPD and ASPD were(91.9±8.3)%and(91.7±10.8)%,respectively.In addition,four figures of merit such as sensitivity(SEN),selectivity(SEL),limit of detection(LOD)and limit of quantification(LOQ)were calculated to further evaluate the performance of the method.The experimental results show that the proposed analysis strategy can be used for direct quantitative analysis of SPD and ASPD of human serum samples in clinic.In Chapter 4:Nephrotic syndrome(NS)is a common clinical syndrome of two main features,increased glomerular basement membrane permeability and decreased glomerular filtration rate,which caused by various reasons.The conventional treatment method is the combined use of immunosuppressive agents and glucocorticoids,which can treat many different pathological types of nephrotic syndrome.However long-term use will produce different degrees of adverse reactions,the drug concentration needs to be monitored during its treatment.At present,the main methods reported in the literature are high performance liquid chromatography for measuring NS treatment drugs,and the pretreatment method is complicated and takes a long time.This chapter uses an alternating trilinear decomposition(ATLD)algorithm combined with high-performance liquid chromatography tandem diode array detector(HPLC-DAD),based on the fast and green“mathematical separation”function of the second-order calibration method to simultaneously and rapidly analyzing prednisolone(PREDL),prednisone(PRED),dexamethasone(DXMS),triptolide(TWP),mycophenolic acid(MPA)and leflunomide(LFM)in the blood.The average recoveries of PRED,PREDL,DXMS,TWP and LFM were(103.5±8.3)%,(101.4±2.6)%,(105.3±7.0)%,(103.6±5.5)%and(91.0±12.4)%,respectively.By monitoring the decay of therapeutic drug concentration in the blood,the metabolic process of the drug in the body can be explored,which can instruct doctors to control the dosage of a single administration in order to personalize the administration plan according to the specific situation of the patient,so as to achieve satisfactory efficacy and avoid the occurrence of poison reaction. |
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