Antioxidant And Anti-fatigue Functional Activities Of Chicken Oligopeptides

Chicken is rich in high quality protein and is a common dish on the family table.Due to the trend of family niche development,most of the current market is the sales model of split chicken,and the chicken breast with poor taste is often ignored by the public and the economic benefits are low.In order to improve the deep processing of chicken breast,improve economic benefits.In this paper,laboratory-made chicken oligopeptides EP1 and EP2 were used as raw materials to measure the oxidation resistance and anti-fatigue of different methods.According to the molecular weight determination,the component having an EP1molecular weight of less than 1000 Da reached 96.99%,and the components having a molecular weight of 180 to 500 Da and 180 Da or less accounted for 57.79%and 30.66%respectively.The molecular weight of EP2 is below 10000 Da,and the components having molecular weights of 180-500 Da and below are 47.70%and 43.45%respectively.The short peptide content of EP1 was 891.67 mg/100 m L,accounting for 69.61%,and the short peptide content of EP2 was 978.94 mg/100 m L,accounting for 56.78%.In the amino acid analysis,the highest content of EP1 and EP2 was glutamine,which reached 49.92 mg/100 m L and111.05 mg/100 m L respectively,accounting for 11.5%and 14.8%respectively.The antioxidant activity of chicken oligopeptides was mainly through four in vitro antioxidant indexes,establishment of cellular antioxidant models and testing of antioxidant indices in vivo.It was found that chicken oligopeptides EP1 and EP2 can effectively improve the total reducing power and the scavenging ability of hydroxyl radicals,superoxide anion and DPPH free radicals,improve the antioxidant capacity of cells,and comprehensively enhance the activity of antioxidant enzymes in vivo.When the concentration was 50 mg/m L,the total reducing power of EP1 and EP2 was 1.92 and 1.55 at 700 nm,and the removal ability of EP1 and EP2 to·OH radicals reached 99.19%and 95.69%,respectively.The scavenging rates of oxyanion radicals were 56.78%and 86.28%,respectively.When the concentration was 10 mg/m L,the DPPH scavenging rates of EP1 and EP2 could reach 98.55%and 95.55%.In Hep G2 cells,EP1 and EP2 were at a concentration of 250μg/m L or less,and the cell viability was above 89%,which was within this concentration range.As the concentration of EP1 and EP2 increases,the fluorescence intensity of the cells is weakened,indicating that both have the ability to inhibit intracellular ROS production,and as the concentration of oligopeptides increases,the antioxidant capacity increases.In the body,in the KM mouse model,the saline,protein homogenate and high and low chicken oligopeptide solution were administered in groups for 28 days.After swimming for30 minutes,the blood was collected from the iliac vein and the serum was determined by the kit.,CAT and GSH-Px vitality.The SOD activity of the low dose group of EP1 and EP2[40mg/kg·bw·d]was increased by 44%and 34%,respectively,compared with the homogenized protein group,and the high dose group of EP1 and EP2[200 mg/kg·bw·d The SOD activity was increased by 50%and 54%,respectively,relative to the homogenized protein group.Compared with the protein homogenate group,the CAT activities of the high dose groups of EP1 and EP2 were increased by 19%and 12%,respectively.Compared with the saline control group,the GSH-Px activity of the high-dose EP1 and EP2 groups increased significantly,increasing by 119%and 118%,respectively.Through the KM mouse model,the fatigue resistance of chicken oligopeptides was studied after 28 days of gavage.Through the exhaustive swimming experiment in mice,it was found that chicken oligopeptide can effectively prolong the exhaustive swimming time of mice.The swimming time of EP1 low-dose group and high-dose group increased by 31%and 201%respectively.The swimming time of the low-dose and high-dose groups was increased by 41%and 170%respectively,compared with the saline blank group.Chicken oligopeptide can effectively increase hepatic glycogen content and reduce the content of lactic acid and urea nitrogen in mouse serum.After swimming for 30 min,both EP1 and EP2in the high and low dose groups could reduce the lactic acid content in the body.The effect of EP1 in the high dose group and the low dose group was extremely significant（P<0.01）,compared with the saline blank group.The lactic acid content was reduced by 33.8%and37.3%respectively.The urea nitrogen content of the high and low dose groups of EP1 was significantly lower than that of the protein homogenate control group（p<0.01）,which was decreased by 34.2%and 24.9%respectively.The urea nitrogen content of the high and low dose groups of EP2 was significantly lower than that of the protein homogenate control group（p<0.01）,which was decreased by 34.8%and 33.0%respectively.In the high-dose group,the hepatic glycogen content of EP1 and EP2 was significantly increased significantly（P<0.01）,which was increased by 49.6%and 59.8%respectively.It is proved that the chicken oligopeptides EP1 and EP2 have even better anti-fatigue effects.Pasteurization（70～80℃,20min）has little effect on the stability and antioxidant activity of oligopeptide solution.The sterilization temperature above 100℃ will reduce the stability of the solution and the antioxidant activity of chicken oligopeptide..Under the condition of p H>6,the antioxidant activity of the oligopeptide is affected.2 m M metal ions such as Fe³⁺and Zn²⁺have certain inhibitory effects on the antioxidant activity of chicken oligopeptides.Therefore,low temperature sterilization should be used in production and processing,and contact with metal vessels such as iron and zinc should be avoided.