| When biological channels are stimulated by external conditions,the selective transmission of ions or molecules is an important way for life to transmit information and exchange materials.The gating effect of biological channels plays an important role in maintaining the operation of life.However,due to the unstable physical and chemical properties of cell membranes,biological channels cannot be effectively used for relevant studies in vitro.In previous studies,functional gated nanochannels were constructed mainly by modifying functional molecules on the surface of nanochannels.Although this method is simple and fast,it has low gating efficiency and single control state.Therefore,it is of great significance and value to select materials with more stable properties and easier operation to construct biomimetic nanochannels for the study of channel gating behavior,and to understand the complex process of information transmission and material exchange in the living body.So how to construct a functional nano-channel with gate effect has become a challenge in the field of nano-channel research.As a new generation of star supramolecules,pillararene have attracted many researchers in supramolecular field for their easy synthesis,functionalization,self-assembly and molecular recognition.Based on this,we designed and synthesized a series of functional pillar[5]/[6]arene molecules as host,designed and synthesized 6 different type of space structure as guest.The pillararene and guest molecules will assemble and inform a complexs in solution phase,and then the host-guest complexes is introduced into the nano-channel,successfully build biomimetic nanometer channel with specific functions.It provides a new way of thinking for nano materials,molecular devices,biological sensors research and so on.The main research contents of this paper are as follows:(1)A variety of chiral amino acids were introduced into the pillar[5]/[6]arene through chemical synthesis to synthesize fully functional chiral pillar[5]/[6]arene.The introduction of chiral amino acids not only enhances the water solubility of pahs,but also,as chiral recognition sites,improves the molecular recognition ability of pillararene,providing a material guarantee for the construction of specific functional biomimetic nano-channels.(2)Inspired by ion gated channels in vivo,bionic nano-channels with high gate efficiency and dynamic regulation were designed and constructed by utilizing the advantages of pillararene and guest molecule self-assembly.During the experiment,negatively charged pillar[5]arene(TP5)and guest molecules(ILG3)were assembled into a complex and introduced into the nanochannel by immersion.Caused by complex into the nano channel,channel internal transport ion effective space is reduced,so that the nano channel show "off" state,when the temperature to 75?,host and guest of intermolecular force is reduced,cause complex dissociation,so that the channel again"on" state.The size of host-guest complex particles is regulated by controlling the time of host-guest assembly,so as to change the effective space of ion transport in the nanochannel and realize the dynamic regulation of the nanochannel.(3)As an inherent property of life,chirality is closely related to many physiological processes in life.Exploring the transport of chiral substances in nano-channels is of great significance not only for the study of the transport process of chiral molecules in living organisms,but also for the transport and separation of chiral molecules.Based on the contents of the previous chapter,we use pillar[6]arene(LAP6)and NIG3 assembly form chiral complex,fill of this host and guest particles in the nano-channel,continue to explore the chirality molecule assembly complex,the influence of the nano-channel transmission and the success of the implementation of R-PPL(propranolol)selective transmission,chiral separation efficiency of ?(R/S)reached 5.80,and successfully realized the separation of racemic PPL,it enantiomer value(e.e.%)reached 33.81%. |
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