Construction Of Selenium-enriched Black Bean Globulin And Polysaccharide Composite Nanocarriers And Release Study Of Ginsenoside Rh2

Selenium-enriched black soybeans are derived from the selenium-enriched industrial base of Jiangxi called “Selenium Valley”.As a new protein resource,the presence of organic units such as selenomethionine(Se Met)and selenocysteine(Se Cys)is the main forms of organic selenium in these selenium-enriched black bean protein,which impart soybean protein and its fractions the new structure,function and nutritional properties.The nano-scale composite system is widely used as a functional system in drugcontrolled carriers.The construction of suitable multi-natural nano-composites has important applied significance for food ingredients,additives and biomedical materials.In this study,we studied the functional properties of selenium-containing soybean fractions and constructed different multi-complexes.By studying their structure and functional properties,some carriers were selected as the embedding system of natural vegetal active extracts,the characteristic of these complexes were studied and the component releasing mechanism was evaluated.(1)In this chapter,we extracted the selenium-enriched black soybean protein fractions and characterized its structural and functional properties at high temperature heatinduced denaturation.It is found that the protein content of Se11 S,amino acid model and the nutritive value is better,and the heat resistance is stronger than Se7 S.The total selenium content of 11 S fraction is higher than the 7S fraction.Electrophoretic analysis revealed that the 11 S protein molecular subunit is smaller,after thermal aggregation,the 7S protein structure was unfolded to some extent,while the 11 S protein tends to form self-assembled particles with heat accumulation.Although the emulsion stability 11 S is slightly less than 7S,the emulsifying activity is significantly higher than 7S,and the gastrointestinal digestion characteristics of Se11 S after heat aggregation are better than 7S,and the gel characteristics are also better.Heated Se11 S is the best fraction to scavenge DPPH and superoxide anion free radicals,indicating that 11 S can be used as a functional protein in the food industry.(2)In this chapter,a binary system and a ternary system between Se11 S protein and chitosan,chitosan-chlorogenic acid conjugation,which is constructed by electrostatic interaction and Maillard glycosylation were formed.The discrepancies in structure and function between these nano-capsules had been studied.The glycosylation system containing polyphenol has the smallest particle size and is relatively stable.The degree of glycosylation of ternary system is slightly higher than that of the polyphenol-free system.All the four systems had formed a gelatinous network structure in microstructure,while the glycosylation system containing polyphenol exhibits a multibranched void structure and contains more hydrophobic microcavities.The infrared spectrum and the nuclear magnetic resonance spectrum proved the existence of free radical aggregation reaction and the presence of molecular forces such as electrostatic interactions,hydrophobic interactions,and covalent bond produced by Maillard glycosylation.Although the emulsifying activity is relatively higher in the polyphenolfree system,the multi-branched structure makes the proteoglycan-polyphenol system a stronger emulsion stability.The proteoglycan-polyphenol system can be an active wall material with better ability to scavenge DPPH and ABTS free radicals.(3)In this chapter,ginsenoside Rh2 was embedded by the nanocarrier containing polyphenol,the structural and functional properties of these nano-systems were evaluated.In this mesoporous gel system,the electrostatic interaction system has a larger particle size and a looser structure,and the mesoporous voids are more conducive to drug entry,resulting in higher loading efficiency and drug loading,and the Maillard system is opposite.The hydrophobic interaction,hydrogen bonding and other intermolecular forces make Rh2 successfully embedded in the hydrophobic microcavity of the nanocarrier,and the protein structure also undergoes a certain degree of remodeling.The Maillard reaction system is more orderly.When concentration of these two drug-loading system and time increases,the release amount and anti-cancer effect are improved.Among them,the electrostatic interaction system is better.Both of them can protect the drug through the gastric phase and reduce the release amount.They are systems that sustained release after burst release,which regulated by p H response and trypsin-mediated.It turns out that the difference in structure causes functional differences.