| Body fatigue is the ability of the body to lose its performance after a lot of exercise.Physical fatigue is associated with a series of physical and biochemical reactions that ultimately affect the person's subjective consciousness,thereby giving up the following actions.Mental fatigue is referred to the psychological pressure is too high,will lead to depression,wandering,and negative,the formation of the overall fatigue mechanism can be divided into four kinds of theories: theory of exhaustion,metabolites block theory,free radical theory,the theory of hemoglobin.Sonchus arvensis L.is not only has rich nutrients,and anti-inflammatory,antibacterial,antioxidant,etc all have more significant effect,formation causes and mechanism of contact fatigue,through the establishment of exhaustion exercise animal models to explore whether Sonchus arvensis L.has anti-fatigue effect.And the products are developed on the basis of the anti-fatigue function to meet the demand of the masses.(1)The measurement of the total flavonoids and total polyphenols in the extracts of Sonchus arvensis L..By spectrophotometric method,draw standard curve,Total flavonoids and total polyphenols were tested for the water extract and alcohols of the Sonchus arvensis L.,so as to explore the function component of Sonchus arvensis L..;The results showed that the contents of the flavonoids in the extract of the Sonchus arvensis L.were 0.685±0.035 mg/g and the total polyphenol content were 0.784±0.005 mg/g.The total flavonoid content in the water extract were 0.91±0.041 mg/g,and the total polyphenol content were 0.793±0.026 mg/g.(2)Research on the fatigue of the extract of Sonchus arvensis L..The experiment was carried out by the design of the inexhaustible motion model,the design of the control group,the exercise group,the exercise + low dosage treatment group and the exercise + high dosage processing group to conduct the animal experiment.Through 30 days of movement,lavage treatment,and finally determine the exhausted time of fatigue in the animals,by comparing and judge whether Sonchus arvensis L.extracts can improve the sport ability of body and relieve fatigue or not.At the same time,various biochemical indexes in the animal are used by means of experimental kits and PCR,such as glycogen,serum urea nitrogen,lactate,hemoglobin,RNA expression quantity,muscle tissue morphology of measurement and observation,so as to determine the fatigue mechanism of Sonchus arvensis L.extract.Results showedthat the intake of Sonchus arvensis L.extract can significantly improve the movement ability of mices,also extend the exhaustion swimming time,it also increases the amount of glycogen and hemoglobin content in the mice and increased the activity of some antioxidant enzymes,the production of metabolites,such as lactic acid and serum urea,also has obvious inhibitory effect.Through the determination of the specific amount of RNA expression in mices,we found that Sonchus arvensis L.extract can effectively increase intake mice CAT and GPX m RNA expression,at the RNA level to enhance the body's antioxidant capacity.To G6 PC and houndreds types product as like GCK and PEPCK gene expression at the same time also has the obvious enhancement effect,that means endives extract intake of the body's metabolism of sugar and sugar dysplasia response has significant regulatory role.(3)The research and development of the product.Considering the overall demand of the sport and the flavor characteristics of Sonchus arvensis L.,the product of Sonchus arvensis L.is developed in the form of beverage.The drinks uses citric acid as an acidic agent,glucose as sweetener,use orthogonal test to optimize beverage ratio,and the best ratio of endives extracts 10% sodium,glucose 12%,citric acid 0.09%.The drink is light yellow,transparent,sweet and sweet,with a unique flavor of Sonchus arvensis L..At the same time,the storage conditions of Sonchus arvensis L.inquiry found that the drink had better be in pasteurization under 4 ? low temperature preservation after sterilization,to ensure the flavor and stability of the beverage. |
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