Investigation On Preparation Of Novel Nanocomposites And Their Application In The Detection Of Hydrogen Peroxide And Glucose

Glucose is an indispensable energy source in organisms,which plays a major role in human's physiological and pathological processes working.In clinical diagnosis,the complications caused by abnormal blood glucose concentration have attracted much attention.Among them,diabetes poses a huge threat to human health,and how to solve it is a worldwide problem.Therefore,the study of glucose sensor with high sensitivity,good selectivity and strong stability has important academic significance and broad application prospects.At present,the blood glucose measurement method used in hospital is mainly enzyme method.However,The use of natural enzymes is easily restricted by the conditions such as rapid inactivation,complex immobilization,poor stability and so on.The continuous development of nanotechnology has opened new windows for developing nanomaterial-based artificial enzymes namely nanozymes to replace natural enzymes.Nanozymes not only have demonstrated catalytic activity resemble that of natural enzymes,but also possess merits including low cost,high environmental stability,and amenability to mass production,which overcome the shortcomings of natural enzyme use in practical applications.Partial nanozymes mimicing the enzymatic activity of HRP,can catalyze the oxidative reaction of various colorimetric substrates by transferring electrons to H₂O₂,and thereby attract significant interest due to their broad application in analytical detection and clinical chemistry.Based on this,the research on preparation and application of novel nanozymes based on magnetic Fe₃O₄nanoparticles aimed at the detection of hydrogen peroxide and glucose was carried out in this paper.The main contents are focused on the follows:1 A hybrid spherical-shell nanomaterial with magnetic Fe₃O₄ nanoparticles as the core loaded with Au-Pt heterodimers outside(the Fe₃O₄@Au-Pt hybrid nanomaterial)was synthesized by seed-mediated growth method in aqueous medium.A simple and sensitive colorimetric sensor based this nanomaterial was constructed for sensitive detection of H₂O₂ and glucose.Experimental results indicated that the Fe₃O₄@Au-Pt hybrid nanomaterial prepared by this method possessed preferable peroxidase-like activity and excellent selectivity for glucose detection in comparison with other nanozymes.For the unique morphology and structure and the synergistic effect among the metal components,this sensor has the characteristics of fast response,high sensitivity and strong anti-interference in glucose detection,and can successfully realize the sensitive detection of H₂O₂ and glucose.This hydrophilic hybrid nanomaterial possesses special structure and excellent catalytic performance,and have low dosage and high sensitivity in the actual blood glucose detection.It is expected to have great application potentials in the sensing analysis of glucose and other biological samples,biomedical diagnosis and treatment in the future.2 A magnetic molecularly imprinted polymer(the Fe₃O₄@GOx magnetic molecularly imprinted microspheres)for recognizing glucose oxidase(GOx)was synthesized by phase inversion process with GOx as template molecule and Poly(vinyl alcohol-co-ethylene)(EVOH)as crosslinking agent in this work.And a simple and sensitive colorimetric sensor was constructed for the detection of glucose based on this nanomaterial.The nano material has strong specific recognition ability for GOx,and is a new type of composite nanozyme.By imprinting the enzymes,the immobilization process of GOx was simplified and the use efficiency was greatly improved.Moreover,in view of the inherent peroxidase-like activity of Fe₃O₄ nanoparticles,this Fe₃O₄@GOx magnetic molecularly imprinted microspheres have dual functions of catalyzing the oxidation of glucose and H₂O₂.The combination of molecular imprinting technology and nanozyme provides a powerful tool for establishing a simple,rapid,green and efficient biological analysis platform.