Dual Manipulation Of Magnetic And Size-based Microfluidic Chip For Three Kinds Of Influenza Virus Detection

In recent years,with the increasing research related to influenza virus in the medical field,researchers have found that point-of-care testing(POCT)can be a key means of suppressing the widespread early spread of influenza virus.Notably,traditional detection methods have several shortcomings: expensive equipment,time-consuming,and the testing environment requirements are relatively high.Therefore,this research focuses on the application status of microfluidic chips in the field of pathogen detection,and is guided by the characteristics of microfluidic chip technology such as miniaturization,high sensitivity,and low reagent consumption,combined with easy-to-control analysis methods such as magnetic fields and the unique fluid phenomenon in microstructure to establish an effective particle manipulation method and virus detection platform.At the same time,we have combined professional strengths with electronics and mechanical engineering technologies to realize the construction of a portable integrated experimental device and the development of an automated humancomputer interaction system,providing realistic development ideas for the application of engineering disciplines in the field of medical devices.the detection platform consumes less reagents,is easy to operate,and has few affected factors.It can effectively realize the switching and application of multiple scenarios,and has certain innovative advantages.In addition,the designed microfluidic chip has a low manufacturing cost,and can be processed and manufactured with a common glass slide structure.By combining the microfluidic chip with molecular diagnostics,the simultaneous detection of three influenza A virus nucleic acids(H1N1,H3N2,H7N9)has been achieved,providing a new detection method for the field of epidemic prevention and control.This project aims at the simultaneous typing detection of H1N1,H3N2,and H7N9 in a microfluidic chip.The main research content is to conduct a series of investigations on the chip detection principle and preparation process,chip function verification,integrated experimental device design,sample processing,and result analysis.The details are as follows:First,microfluidics chip controlled by magnetic fields and spatial structures were prepared using corresponding microfabrication techniques.Secondly,the mixed solution of microspheres(magnetic beads and polystyrene spheres)of different sizes is passed into the sample chip.Under the dual control of the magnetic field/spatial structure,the separation and capture of the microspheres in the microfluidic chip are realized.Then,a portable hardware experimental device based on ARM chip as the control core system is developed and built with the cooperation of embedded electronics,machining technology and 3D printing technology.Finally,through the design and implementation of the experimental scheme,combined with the luminescence characteristics of quantum dots(SA-QDs)under the fluorescence microscope,using the principle of nucleic acid specific hybridization,the fluorescence image of the specific nucleic acid hybrid complex captured in the sample chip was quantitatively analyzed to achieve simultaneous detection of H1N1,H3N2,and H7N9 in a microfluidic chip.