Branched Chain Amino Acid Aminotransferase-1 Regulates Adipose-derived Mesenchymal Stem Cell Survival Via P53 Signaling

Objective:To determine the role of branched chain amino acid aminotransferase 1(BCAT1)in the survival of mesenchymal stem cells(MSCs);to explore the mechanism of BCAT1regulating MSCs survival.Methods:ADSCs were isolated from white adipose tissues of male Sprague Dawley rats.BCAT1 expression was knocked down or overexpressed in ADSCs by adenovirus transfection.ADSCs apoptosis was induced by hydrogen dioxide(H₂O₂)stimulation.ADSCs apoptosis was evaluated by CCK-8 cell viability assay,cleaved caspase-3expression,and TUNEL staining.The activity of P53 was suppressed by the specific inhibitor PFT-?in control or BCAT1 silencing ADSCs.Results:BCAT1,rather than BCAT2,was the predominant subtype expressed in ADSCs.Silencing of BCAT1 exacerbated,whereas BCAT1 overexpression ameliorated hydrogen dioxide-induced ADSCs apoptosis.The downstream targets of P53 were significantly increased in BCAT1 knock-downed ADSCs when stimulated by hydrogen dioxide.Inhibition of P53 by its specific inhibitor PFT-?reversed the adverse impact of BCAT1silencing on ADSCs survival.Conclusion:BCAT1 regulates ADSCs survival via a P53-dependent manner,revealing that BCAT1 is a promising target to enhance ADSCs survival and their cardioprotective efficacy.