| In the field of molecular biology,RNA triplex poly(U)(9)poly(A)·poly(U),plays an important role in many biological functions,has been discovered.However,the weak stability of the RNA triplex Hoogsteen hydrogen bond will lead to the instability of the entire structure,which limits its application in biological functions.According to the current research results,ruthenium(II)metal complexes are used as intercalantors to bind to the RNA triplex,and regulate the binding stability of the third strand,which has great potential for development.Therefore,this paper designs some new complexes with ruthenium as the central atom as intercalantors to regulate the stability of the RNA triplex.First,two chirality Ru(II)polypyridine complexes?-[Ru(bpy)2(6-NO2-dppz)]2+(?-1)and?-[Ru(bpy)2(6-NO2-dppz)]2+(?-1)have been design and synthesis.The results of the study show that?-1 and?-1 are intercalated into the RNA triplex,and binding affinity of?-1 is slightly greater than that of?-1.The two enantiomers have no obvious selectivity for the stabilization of the RNA triplex.Secondary,we designed and synthesized two complexes with different main ligands structures[Ru(bpy)2(5-idip)]2+(Ru-2)and[Ru(bpy)2(biip)]2+(Ru-3)and the main ligand tails of Ru-2 and Ru-3 are indole and benzimidazole,respectively.The results showed that the binding mode of Ru-2,Ru-3 with the RNA triplex is intercalative binding,while the binding affinity of Ru-3 is much greater than that of Ru-2,and Ru-3 has a great stabilizing effect on the RNA triplex than Ru-2.Ru-3 is significantly stabilizing effect on third strand and template duplex without obvious selectively,while Ru-2selectively stabilizes the third strand with only a single broad transition.The previously studied Ru-2 has the effect of selectively stabilizing the third strand of the RNA triplex,so the chiral?-2 and?-2 with the same structure as Ru-2 has been synthesized.The binding modes of the two enantiomers with the RNA triplex are intercalation modes,the binding strength of?-2 with the RNA triplex is greater than?-2.Meantime,two enantiomers are stable the third strand and template duplex of the RNA triplex,while tend to stabilize the third strand.?-2 and?-2 have no obvious difference in the stability of the RNA triplex,which are different from Ru-2.Ru-3 has a good stabilizing effect on the RNA triplex,so the binding properties of different chirality Ru-3 with the RNA triplex were studied.The results of the study showed that the binding mode of the two enantiomers with the RNA triplex is intercalation modes,while the binding strength of?-3 is slightly greater than that of?-3.Meantime,thermal stability experiments show that two enantiomers can better stabilize the third strand and the template duplex,while tend to stabilize the third strand,and the stabilizing effect of?-3 is better than that of?-3,which differ from Ru-3.Finally,Ru(II)polypyridine complexes with different auxiliary ligands[Ru(bpy)2(iip)]2+(Ru-4),[Ru(phen)2(iip)]2+(Ru-5)has been synthesized and studied.The results show that binding modes of Ru-4,Ru-5 with the RNA triplex are intercalation modes,and binding affinity of Ru-5 is slightly greater than that of Ru-4.Two complexes can stabilize the RNA triplex,Ru-4 effectively improves stability of the third strand and template duplex,it is more inclined to stabilize the third strand,while Ru-5 is selectively stabilize the third strand and has no effect on the template duplex. |
PDF Full Text Request