| Cancer is the biggest threat to human health.With the development of economy,people's living standards and modern medical standards have been improved.Attentions to health have been gradually increased.People hope to find diseases and treat them in time in the early stage of cancer,so as to improve the cure rate.At present,the existing detection methods can't detect biomarkers at ultralow concentrations in the early stage of cancers,many cancers go undiagnosed until they are in the advanced stages.Therefore,it is necessary to continuously improve the sensitivity of sensors.In recent years,optical microfiber biosensors have been developed rapidly.Its characteristics of light weight,strong anti-electromagnetic interference and low cost provide greater possibilities for the application in early detection.Optical Micro-fiber biosensor is composed of excitation light source,fiber sensor probe and spectrometer(OSA)The detection performance of the sensor is mainly determined by the probe.In previous studies,sensors have low sensitivity and cannot be widely used in biomedical and environmental detection.Therefore,improving the sensitivity of sensor is more conducive to accurate analysis of biochemical reaction process.Nanomaterials is found to be well compatible with biological cells.The combination of nanomaterials and optical fibers can also improve the detection limit of sensors.In addition,the integrated targeting therapy function of nanomaterials on the micro-fiber sensor interface is more targeted than the current nanotherapy methods,which can kill cancer cells without damaging normal cells,and realize the real-time evaluation of curative effect.Based on the above,the following contents are studied in this paper:1.We constructed a nano-interface composed of gold nanorods(Au NRs)supported by BP nanosheets on an optical microfiber to integrate ultra-sensitive biosensing and precise nanotherapy functions.Here,we use breast cancer cell lines as a cancer model.Human epidermal growth factor receptor 2(HER2),a key prognostic protein biomarker for the treatment of breast cancer patients,is selected as the detection target.BP nanosheets are loaded with the anti-cancer drugs doxorubicin(DOX),which are used to establish a drug delivery platform for cancer chemotherapy.At the same time,in order to optimize the plasmon electromagnetic field enhancement,the BP nanosheets are used as spacers to support Au NRs.The Au NRs on the BP nanosheets improve the sensitivity of the microfiber through the enhancing the evanescent field.The detection limit(LOD)of sensor achieves 0.89 aM,which can detect the biomarkers at ultralow concentrations in the early breast cancer.2.The nano-interface integrates the targeted therapy function on the micro-fiber,and can evaluate the treatment results in real time.Using the synergistic photothermal effect of Au NRs and BP,the cancer cells captured on the optical fiber can be killed by photothermal effect.The drug release of DOX can also be triggered by the photothermal effect,and kills target breast cancer cells.At the same time,the sensor can realize real-time evaluation of therapeutic effect by real-time monitoring of HER2 molecules.In this process,photothermal reagents and DOX are integrated on the fiberoptic nanointerface,which can avoid the damage to normal cells caused by the enrichment of nanomaterials.These findings provide an easy-to-use alternative for the integration of early in situ diagnosis,nanotherapy and post-healing evaluation. |
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