Description Of Six Species Of Bopyrinae(Crustacea: Isopoda) And Analysis Of The Complete Mitochondrial Genome Of The World Wide Species-Bopyroides Hippolytes(kr(?)yer,1838)

Subfamily Bopyrinae belongs to the phylum Arthropoda,subphylum Crustacea,class Malacostraca,order Isopoda,suborder Epicaridea,family Bopyridae.The adults are mainly parasitic in the branchial cavity of Caridea(Decapoda),and their hosts are mainly economic shrimps of the Alpheoidea and Palaemonoidea.A total of 27 genera and 127 species of the Bopyrinae have been identified worldwide.However,the geographical distribution,host situation and phylogeny of this subfamily have not been reported.(1)Taxonomic study: This paper describes in detail six genera and six species(two new species and one newly recorded species)of the Bopyrinae in the Philippines and other seas.And summarizes the genera in which they are found.The identification characters of male and female individuals of each species are described,key to the species of five genera was also prepared.(2)Geographical distribution and hosts: The geographical distribution of 127 species of the Bopyrinae was analyzed,and the results showed that,except for Bopyroides hippolytes(Kr?yer,1838),which is distributed in the cold waters of the Arctic Ocean.The rest of the species are mainly distributed in the Indo-Pacific range,mostly in Japan,India,the United States Gulf,Caribbean,the Gulf of Mexico,Thailand,Indonesia;Chinese waters are mostly found in Hong Kong,Taiwan,Qingdao,Hainan and other coastal cities.The hosts of species of the Bopyrinae are mainly concentrated in the general family of the Alpheoidea(about 74%),it can also be parasitic on Palaemonoidea,Pandaloidea and Processoidea.(3)Mitochondrial genome: Molecular data for this taxon are extremely scarce,with Cox1 sequences for only two species of the Bopyrinae,and without complete mitochondrial data are available at this time.In this paper,we obtained for the first time the complete mitochondrial genome sequence of B.hippolytes in Bopyrinae.The genome of B.hippolytes is comprised of15,329 bp and 34 genes,with 13 protein coding,19 transfer RNA(t RNA-I,t RNA-W and t RNAK missing),2 ribosomal RNA genes.The A+T content is 64.64%.The DHU arm was missing in t RNA-C,while the T?C arm was missing in t RNA-G.B.hippolytes has two ribosomal genes,12 S and 16 S.12S(829 bp)is located in the(+)chain,while 16S(1172 bp)is located in the(-)chain.The B.hippolytes mitogenome include 10 intergenic regions(1153 bp)and 13 overlapping regions(80 bp).(4)Phylogenetic analysis: In this paper,four mitochondrial genes and 18 S r RNA of Parabopyrella mortenseni(Nierstrasz & Brender à Brandis,1929),Bopyrella malensis Bourdon,1980 were sequenced.On this basis,the phylogenetic tree was constructed by combining the molecular data in the Gen Bank database,preliminary exploration of the affinities of this subfamily with other subfamilies in Bopyridae.The results show that at the suborder level,Phreatoicidea is sister to all other isopod species;suborder Limnoriidea and the Epicaridea form a sister branch.At the subfamily level,Athelginae + Pseudioninae forms sister groups with other subfamilies.Argeiinae and Bopyrinae are more closely related.