Construction And Expression Of Eukaryotic Expression Plasmid Vector FGFR1-MIP3α/Fc Fusion Gene In Vitro

Objective: To construct and express a eukaryotic expression plasmid of mice FGFR1-MIP3α/Fc fusion gene,and detect its expression in the cells of 293 T which to provide laboratory data for the study of FGFR1-MIP3α/Fc fusion protein against tumor angiogenesis.Methods: The FGFR1-MIP3α fusion gene fragment was amplified by PCR with primers;After digesting by restriction endonuclease,the FGFR1-MIP3α fusion gene fragment was inserted into the eukaryotic expression vector of pc DNA3.1(+)/Fc(Mouse IgG2a)to construct the expression plasmid of FGFR1-MIP3α/Fc;After confirming by PCR,enzyme digestion analysis,and sequencing,the recombinant plasmid of FGFR1-MIP3α/Fc was transferred into 293 T cells and the expression of the fusion gene was identified by ELISA.Results: The digestion by PCR,restriction endonuclease and DNA sequencing demonstrated that the recombinant plasmid FGFR1-MIP3α/Fc fusion gene was correctly constructed;the plasmid could expressed FGFR1-MIP3α/Fc protein in 293 T cells by ELISA.Conclusion: The eukaryotic expression Plasmid of FGFR1-MIP3α/Fc was successfully constructed and expressed in 293 T cells.