Studies On The Microbiome Characteristics Of Sweat-stained Sportswear

Objective: To study the basic microbiological characteristics of polyester sportswear producing sweat odor.By comparing the microbial flora in sportswear fabrics with other materials(pure cotton,blended fabrics),to clarify the specificity of microorganisms in polyester sportswear.It provides basic microbiological data for targeted elimination of odors.Methods: We selected 10 volunteers who live in Qiaokou District of Wuhan and meet the eligibility requirements.Each volunteer wore a sweatshirt made of polyester,cotton,and blended materials,and exercised to the point of visibly sweating.After the sportswear was sealed in the dark at room temperature for 72 hours,the microbial flora DNA was extracted.We used Illumina high-throughput sequencing technology(Nova Seq Illumina sequencing platform)to sequence and analyze the V3-V4 hypervariable region of the 16 S r RNA gene in the bacterial flora in each sample.After quality control of the sequencing results,the diversity and relative abundance of the sample flora were analyzed,and the flora characteristics of polyester sportswear were statistically analyzed.The P values of Alpha diversity and Beta diversity analysis were obtained by Kruskal-Wallis test and PERMANOVA test,respectively.We used Fisher's exact probability method to test the difference in the detection rate of the same genus in polyester,cotton and blended sportswear and used the Kruskal-Wallis test to analyze the statistical difference in relative abundance.Results: 36 samples were sequenced,resulting in a total of 4,452,520 original sequencing sequences.After quality control,a high-quality sequence 4283544(accounting for 96.20% of the total readings)for subsequent analysis was obtained.In this study,99% similarity was used as the dividing standard,and a total of 13,942 amplified sequence variants were obtained.The samples detected 3 domains,41 phyla,88 classes,210 orders,354 families,and 802 genera of microorganisms.The rarefaction curve calculated by Shannon index showed that at this sequencing depth,all species in each sample had been covered.There were no significant differences in ? diversity and? diversity among sportswear of different materials.The difference in the detection rate and in relative abundance of 48 species of bacteria with relative abundance greater than1% in polyester,pure cotton and blended sportswear were more consistent.The detection rate and relative abundance of Pseudomonas in the polyester experimental group were higher than those in the pure cotton experimental group(P < 0.05)and the blended experimental group(P < 0.05).The relative abundance of Enhydrobacter in the polyester experimental group(P < 0.05)and the blended experimental group(P < 0.05)was lower than that of the pure cotton experimental group.The relative abundance of Corynebacterium in the polyester experimental group was lower than that in the pure cotton experimental group(P < 0.05).The relative abundance of Pantoea in the blended experimental group was higher than that in the pure cotton experimental group(P <0.05).Conclusions: The genus of sportswear bacteria of different materials has particularity in detection rate and relative abundance: the detection rate and relative abundance of Pseudomonas in polyester sportswear are higher than those of pure cotton and blended sportswear;the relative abundance of Enhydrobacter in polyester and blended sportswear is lower than that of pure cotton sportswear;the relative abundance of Corynebacterium in polyester sportswear is lower than that of pure cotton sportswear;the relative abundance of Pantoea in blended sportswear is higher than that in pure cotton sportswear;the colonization characteristics of bacteria on clothing may be related to the peculiar smell of perspiration.