| In recent years,the plasma discipline has gradually emerged as an emerging discipline and has been applied in various fields.Among them,the development of plasma medicine is the most promising.Plasma biomedical applications are mainly embodied in plasma sterilization and plasma treatment of tumor cells.In the development of plasma medical applications,the issue of plasma dose has not been widely accepted scientifically.Since RONS is the key active particle that dominates the biological effects of plasma,they play a key role in plasma treatment of bacteria and other aspects,and play an important role in the pathological process of cells,so it is natural to define plasma dose based on RONS.select.Therefore,this paper proposes to define the plasma dose based on the total oxidation potential of plasma active particles,and uses the antibacterial effect as the biological effect evaluation of the plasma,and conducts research in the following aspects:(1)First,define the total oxidation potential of the plasma active particles,and combine the existing measurement and concentration results of the active particles to simplify the model,and then establish the calculation model of the plasma total oxidation potential and antibacterial effect.(2)Research and summarize the sterilization results of the two internationally-used plasma sterilization devices and the diagnostic data of their active particles,obtain the antibacterial effect data from the literature,and bring them into the calculation model to obtain the antibacterial effect.Further investigating the literature to obtain the density of active particles,and bring the total oxidation potential calculation model into the total oxidation potential results.The two results are curve-fitted to verify that there is a linear relationship between the two,and to illustrate the feasibility of the total oxidation potential to define the plasma dose.(3)Independently research and develop a pulse-excited plasma jet generator,and further verify the feasibility of the total oxidation potential to define the plasma dose through experiments.The main part of the discharge of the device is composed of a quartz tube and a copper ring electrode.The excitation source is a pulse voltage with an amplitude of 8 k V and a frequency of 8 k Hz,respectively in He,He+0.5%O2,He+1%O2 and He+0.2%H2O in these 4 different working gases,the time gradients of 5,8,10,and 15 minutes are treated with plasma respectively.The results show that the device has the best sterilization effect in pure He and He+0.2%H2O plasma,and the BRF is reduced by about 4 orders of magnitude at 15 minutes,while under the two conditions of He+0.5%O2 and He+1%O2 After 15 minutes,the BRF decreased by only 2.26 and 1.10 orders of magnitude.(4)Diagnose the concentration of short-lived key active particles(OH and O)by laser-induced fluorescence method,measure the concentration of O3,NO,NO2 by absorption spectroscopy,measure the density of N2O,1O2by numerical simulation method,and find OH by measurement The density is 1019/m3?1021/m3,the density of O is 1021/m3,the concentration of O3 is 1021/m3?1022/m3,the concentration of NO is 1019/m3?1020/m3,and the concentration of NO2 is 1022/m3?1023/m3,the density of N2O is 1018/m3,and the density of 1O2is 1016/m3.After substituting the formula,the total oxidation potential is 5.50×1019e V?3.09×1020e V.Finally,the lg ETOP and BRF are fitted to prove that the two obviously linear relationship. |
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