| Glucose metabolism occupies an extremely important position in the body’s metabolism.The energy required for human life activities is mainly provided by glucose metabolism.Carbohydrate metabolites are the main energy materials of living organisms.Disorders of metabolism can lead to diseases.Therefore,the detection of glucose metabolite products is very important in disease monitoring.Photoelectrochemical sensors have the advantages of low background interference,fast response,high sensitivity,low cost,and easy miniaturization.They have been widely used in the detection of heavy metal ions,chemical small molecules,biological macromolecules,cells and other analytical fields.However,similar to electrochemical sensors,photoelectrochemical sensors still have disadvantages such as poor reproducibility and poor stability.Therefore,the goal of this paper is to develop a new type of anti-interference photoelectrochemical sensor to improve the reproducibility and stability of the sensor,and to establish a new method for the detection of glucose metabolite products.In this paper,we developed a dual-signal mode ratiometric photoelectrochemical sensor for glucose determination by using poly-（neutral red）（PNR）and Zn In2S4nanoflower as photosensitive materials.They were located at adjacent areas on one electrode substrate.By applying different bias voltages,both signals can be well separated without mutual interference.The photoelectrochemical response signals for glucose were recorded by chronoamperometry,and showed a wide linear range from0.08 m M to 30 m M（R2=0.997）with a detection limit（S/N=3）of 27μM.The fabricated PEC sensor also demonstrated excellent selectivity,favorable reproducibility and long-term stability for glucose detection due to the specificity of enzyme and dual ratiometric signal mode.Moreover,this photoelectrochemical ratiometric sensor was successfully used to measure glucose level in human serum samples,and the results agreed well with those from commercial glucose meter.In this thesis,an anti-interference photoelectrochemical sensor with separated photoanode and biocathode was constructed.The Zn In2S4/FTO（Fluorine-Doped Sn O2Conductive Glass）electrode was used as a photoanode to generate a photoelectric response signal,and the NPG/FTO electrode with an enzyme as a recognition element was used as a biocathode to achieve specific recognition of the detection object.The detection sample is specifically identified at biocathode,so that the coexisting reducing substances do not affect the photoanode interface,thereby effectively reducing the influence of interfering substances on the photocurrent signal at photoanode.Under optimal conditions,the photoelectrochemical response signal has a good linear relationship with lactic acid in the concentration range of 1μM～10 m M,and the detection limit is 0.23μM;it has a good linear relationship with pyruvate in the concentration range of 10μM～5 m M,the detection limit is 2.73μM.In addition,the feasibility was verified by using an ammeter to replace the electrochemical workstation as the signal output terminal.It provides a new idea for the subsequent development and design of miniaturized,low-cost,and portable photoelectrochemical sensing devices,which has good reference significance.In summary,this work focus on improving the stability,reproducibility,repeatability and anti-interference performance of the photoelectrochemical sensor through the construction of the ratio sensor and the bipolar separation sensor.At the same time,it provides a new research idea for the miniaturization and commercialization of sensors,laying a certain work foundation.It provides a fast and accurate analysis method for disease monitoring and biological analysis. |
