| Endophytic fungi refer to fungi which exist in healthy plant tissue at a certain stage in the history of life,do not cause obvious symptoms of host or cause obvious harm to host,mainly composed of Ascomycete fungi and their stage of asexual,also include a small number of Basidiomycetes and Zygomycetes.Endophytic fungi can also be understood as normal flora in plant tissue,and can be separated from the tissue with strict surface disinfection or can directly amplify the DNA in the tissue to study.Tree fern Alsophila spinulosa is an endangered relic plant in the world.The research on endophytic fungi in A.spinulosa has not been reported yet.Here,the diversity and community composition of endophytic fungi in A.spinulosa were investigated based on morphological characteristics and phylogenetic analyses of DNA sequence data.Our results revealed that A.spinulosa concealed abundant fungal species,and the colonization rate of endophytic fungi reached 87.6%.A total of 291 isolates of endophytic fungi belonging to 64 different taxa were obtained from 234 living tissue segments of A.spinulosa.Approximately 60% were common species,and Nemania diffusa exhibited predominantly.The diversity index of endophytic fungi was as high as 3.53 and varied with different microhabitats ranged from high to low in rachis > root > pinna > petiole > bark.The species composition and distribution of endophytic fungi in the plants might be affected by the tissue properties of A.spinulosa,as indicated by the analysis of cluster and Sorenson's similarity coefficient.More than 80% of endophytic fungi in the A.spinulosa sample had been isolated according to the analyses of species accumulation curves.Our result could reflect the diversity and community composition of the fungi.Analysis results of colonization rate,species richness,diversity,and community similarity in the petiole litter of A.spinulosa indicated that the community of fungi in the litter had changed considerably. |
PDF Full Text Request