Design Of Real-time Cell Temperature Monitoring System And Temperature Monitoring Of Neural Stem Cells

Neural stem cells(NSCs)are a type of cells that can differentiate into nerve cells,astrocytes,and oligodendrocytes.They have self-renewal capabilities.Based on their proliferation and differentiation characteristics,NSCs have broad application prospects in promoting the repair of damaged central nervous system.Although there are some studies to understand the proliferation and differentiation,there are few reports on the research of temperature signals during its growth.It has been reported that neural stem cells and differentiated progeny cells have different metabolic states.Temperature monitoring may provide reference information for NSCs differentiation status and metabolic transformation.In this thesis,based on wireless multi-channel detection equipment,a real-time temperature monitoring system is built to monitor the temperature of NSCs during adherent growth.Furthermore,the thyroid hormones are interacted with NSCs,monitor the temperature changes of NSCs,and infer its effects on the metabolism of NSCs.The main content of the thesis is as follows:Firstly,a wireless system for real-time monitoring the temperature of cells during adherent growth was designed.The system uses a thin-film platinum thermal resistance as a temperature sensor.The resistance detection device uses a battery to supply power and uses Bluetooth to transmit data.The cell culture device is placed into the incubator to ensure that the neural stem cells grow normally during the temperature monitoring process.The host data processing system monitors and records the temperature changes of the neural stem cells.The temperature resolution of this system is 0.05 °C,There are12 detection channels to work continuously for more than 24 hours.Secondly,the NSCs were extracted from the brain of fetal rats to culture.After coating the film platinum thermal resistance surface with rat tail collagen,the NSCs were induced to adhere to the resistance surface.the designed temperature monitoring system was applied to monitor the cell temperature in real-time.The results showed that when 5000 ± 300 neural stem cells were attached to the resistance surface,the cell temperature is increased about 0.3°C due to the adherence behavior.After attachment,the temperature is kept stable for the growth of neural stem cells.Finally,the triiodothyronine(T3)was added to intervene with neural stem cells.The results indicate that obvious and rapid temperature increase is observed once the addition of T3 in the cell culture.When the concentration of T3 was 0.1 ?mol/L,the temperature of neural stem cell population increased by 0.15°C,and when the concentration of T3 was 1 ?mol/L,the temperature of neural stem cell population increased by 0.4°C.Furthermore,by correlating the biological process of thyroid hormone to promote the body's heat production,the biological principles of the temperature changes of neural stem cells mentioned above were explored,and the possible mechanism of T3's regulation of the transformation of NSCs' metabolism by regulating gene expression and promoting mitochondrial division was discussed.