Function Analysis Of Protein SCO5477 In Streptomyces Coelicolor

As one of model strain of Streptomyces,Streptomyces coelicolor has always been a research hotspot for its morphological differentiation and secondary metabolism.In recent years,with the rapid development of proteomics,the important role of transporters and transport systems related to morphological differentiation in Streptomyces coelicolor has got much attention of researchers.In addition,different transport systems also play a vital role in the transport of secondary metabolites such as antibiotics.Therefore,the study of transport proteins and transport systems has great application prospects in the industrial production of Streptomyces coelicolor.Based on the related research results of SCO4114,the function of SCO4114 interacting proteins was firstly analyzed,and the direct interaction between each protein and SCO4114 was verified by SPR.The results showed that SCO4114 can directly bind to multiple proteins such as SCO5477,SCO2008 and SCO2180,further affecting energy metabolism,protein synthesis,and material transport,Among them,SCO5477 bind to SCO4114 most tightly.SCO5477,an oligopeptide binding protein(Oligopeptide binding protein),is an important part of the ABC transport system and can participate in the important life activities of Streptomyces coelicolor.Therefore,SCO4114 may mainly regulate the transport function of SCO5477,and then affect secondary metabolic processes such as morphological differentiation and sporulation.Furthermore,functional analysis of protein SCO5477 was conducted.The experimental results showed that the growth rate of (?)sco5477 muntant was accelerated,and the production of two types of antibiotics,actinomycin(ACT)and undecyl aurein(RED),has decreased.The spores of the mutant mycelium matured earlier.Therefore,SCO5477 as an oligopeptide transporter can affect the sporulation and secondary metabolic processes of Streptomyces coelicolor.Scanning electron microscopy(SEM)observed that the spores matured earlier.Therefore,SCO5477 may affect the sporulation and secondary metabolic processes of Streptomyces coelicoloras an oligopeptide transporter.The results of this study show that the SCO4114 protein in Streptomyces coelicolor can regulate the transport function of the oligopeptide binding protein SCO5477,and that SCO5477,as an important part of the sporulation metabolic regulation network of Streptomyces coelicolor,can not only participate in the primary metabolic process,but also has a great relationship with the synthesis of antibiotics.Therefore,the in-depth study of the oligopeptide transporter SCO5477 can provide an important experimental basis for the construction of morphological differentiation network and industrial application of Streptomyces coelicolor.