Investigation On Cell Growth Performance Of Microfluidic Chip Surface Regulated By Laser

After years of development,microfluidic technology plays an irreplaceable role in the research of cell biology and cell analysis.However,the microfluidic chip,which integrates various functions of chemical analysis into a chip of several square centimeters,is the most active development front at present,which represents the development direction of miniaturization and integration of analytical instruments in the 21 st century.Due to its low cost and many good properties,polydimethylsiloxane has gradually become a good choice for conventional microfluidic chips and structural materials used as cell culture chips.Based on the above advantages and a wide range of practicability,polydimethylsiloxane is the microfluidic chip material selection of this research.Then,nanosecond laser technology is used to regulate and control the PDMS surface structure and cell growth in an efficient,fast and low-cost processing way.The following are the specific research contents.Firstly,the theoretical model of laser-polymer interaction and tensile corrugated model are put forward,and this theoretical basis is provided for the subsequent experimental research and simulation verification.Secondly,nanosecond infrared laser thermal induction technology is used as the processing method.The waveforms were detected and analyzed by atomic force microscopy(AFM),scanning electron microscopy(SEM)and Raman spectrograph.The relationship between the process parameters(laser power density and pre-strain provided by the die)and the wavelength and amplitude of the corrugated structure on the surface of PDMS was found.The adjustable range values of the wavelength and amplitude of the width corrugated structure with the shape characteristics of the linear structure are found.The results show that the chemical structure of the modified surface does not change and the Young's modulus of mechanical properties will increase with the increase of laser power density.Thirdly,COMSOL finite element software was used to simulate the temperature field module of the experimental model.Through numerical calculation of the model,the thickness of the hard layer induced by laser heat induced PDMS was calculated.Combined with the measurement results of Young's modulus of corrugated surface by AFM atomic mirror,the theoretical value of the theoretical model is obtained through the calculation of the theoretical model.Forthly,the effects of corrugated structure and infrared nanosecond laser thermal induction on the growth of PDMS cells were studied and verified.