Analysis Of The Differential Expression And Regulatory Network Of Long Non-coding RNAs Under The Abiotic Stresses In Drosophila Melanogaster

To adapt to constantly changing natural environment,organisms developed complex stress-response system.Previous researches mainly focused on the proteincoding genes under abiotic stresses,while the information of long non-codingRNA(lncRNA)is limited.In this study,Drosophila melanogaster was used to study the stress response under abiotic stresses from the perspective of long non-coding genes.Based on theRNA-seq sequencing data of D.melanogaster under abiotic stresses,stressrelated differentially expressed lncRNA genes were sought out,and co-expression network of these lncRNAs was constructed,and hub lncRNAs in networks were found.The results of this study are as follows:1.Analysis of abiotic stress transcriptome assembly and differentially expressed lncRNA in D.melanogaster910 libraries of D.melanogaster,which are related to abiotic stresses such as diet,temperature,toxic substances and light cycle treatment,in the National Center for Biotechnology Information database,were downloaded and assembled.A total of17,392 genes were identified and 3,612 of them were lncRNAs,including 1,179 novel lncRNA genes.LncRNAs involved in stress response in D.melanogaster under abiotic stresses were identified by differential expression analysis.Under dietary nutritional imbalance stress,402 lncRNAs were significantly up-regulated and 270 lncRNAs were significantly down-regulated.Under temperature stress,1,500 lncRNAs were significantly up-regulated and 1,548 lncRNAs were significantly down-regulated.Under stress of toxic substances,45 lncRNAs were significantly up-regulated and 426 lncRNAs were significantly down-regulated.Under light cycle imbalance,there was only 1 lncRNA significantly down-regulated.It indicates that lncRNAs are involved in the regulation of abiotic stress response in D.melanogaster.2.Regulatory network analysis of stress-related lncRNAsUsing weighted gene co-expression network analysis,7,2,and 2 modules were highly related to starvation,temperature and radiation treatments.The protein coding genes related to stress-related lncRNAs were selected for GO function annotation and KEGG pathway enrichment analysis.The results indicated that under starvation stress,genes were significantly enriched in carbon metabolism,phagosome and lysosome pathways,longevity regulation pathways,detection of chemical stimulus and cells signal pathways related to proliferation and apoptosis.Under low temperature stress,genes were significantly enriched in the phosphatidylinositol signaling system,Hippo signaling pathway,carbon metabolism,glycolysis,phagosome pathways.Under high dose radiation exposure,genes were significantly enriched in the carbon metabolism pathway and participate in biological processes such as chemical stimulation perception and tRNA aminoacylation.It indicates that stress-related lncRNA involved in energy metabolism,signal transduction,cell proliferation,and apoptosis.It is closely related to the external perception,internal metabolism and overall homeostasis of D.melanogaster under adverse environment,and plays an important role in the regulation of stress response.3.Hub lncRNA selection and network constructionThe hub lncRNAs which play a key role in regulation of stress response were selected.In the stress regulatory network of starvation,low temperature,and radiation stress,98,135,and 8 hub lncRNAs were found,respectively.The hub lncRNA and protein coding genes constitute together to form a complex regulatory network under abiotic stresses in D.melanogaster,providing valuable data resources for studying the mechanism of non-codingRNA regulation of abiotic stress response.