Effect Of Lactobacillus Plantarum P9 On Fecal Bile Acid And Short-chain Fatty Acids Metabolism Of Populations Exposed To Pesticide

Probiotics can promote body health by promoting the expression of key enzymes in the intestine,improving intestinal barrier function and regulating body immunity to reduce the damage of pesticide residues to body.Lactobacillus plantarum P9 has been proved to be effective in alleviating the phorate poisoning in model rats,improving the degradation rate of pesticides in the intestinal tract,and promoting the steady state of intestinal flora.On account of this,a random and controlled experiment was conducted in 70 volunteers exposed to pesticide in agricultural areas.The population was divided into the low exposure placebo group(A1),the low exposure probiotic group(A2),the high exposure placebo group(B1)and the high exposure probiotic group(B2).A total of 140 fecal samples collected on 0 days(Day 0)and 30 days(Day 30)were used as the research subjects.The detection method of BAs and SCFAs in feces was optimized and targeted quantitative detection was conducted by UPLC-MS/MS technology to explore the effects of Lactobacillus plantarum P9 on the metabolism of BAs and SCFAs in feces of people exposed to pesticides.The main results were as follows:(1)The fecal BAs and SCFAs metabolism of people with low exposure to pesticides(A1 and A2)changed little after intake of Lactobacillus plantarum P9 or placebo for Day30,and there was no significant change within Day 30(P>0.05).(2)After intake of Lactobacillus plantarum P9 or placebo for Day 30,the fecal BAs metabolism of people with high exposure to pesticides(B1 and B2)changed in different degrees.The contents of CA,CDCA,DCA and LCA increased significantly in B2(P<0.05),but there was no significant change in B1.In the people exposed to pesticides with high initial BAs content,CA and DCA in B1 and DCA in B2 decreased significantly(P<0.05),which could promote the balance of BAs metabolism.(3)After intake of Lactobacillus plantarum P9 or placebo for Day 30,the changes of SCFAs content in B1 and B2 were similar to those of BAs metabolism,and there were people with rising content and falling content.The changes of SCFAs in B2 were more significant than those in B1(P<0.05).The contents of acetic acid,propionic acid,butyric acid,valeric acid and caproic acid in B1,valeric acid and caproic acid in B2 were all decreased significantly(P<0.05).This difference may be related to the individual's diet or physical condition.Through this study,it was found that low exposure to pesticides had little effect on the metabolism of fecal BAs and SCFAs,while high exposure to pesticides affected the metabolism of fecal BAs and SCFAs.Lactobacillus plantarum P9 can alleviate the effects of high exposure to pesticides on the metabolic balance of BAs and SCFAs,and dietary habits or health level have an important impact on them.