Research On Hyperspectral Polarization Microscopy For Multi-dimensional Bio-optical Information Sensing

The photons emitted from the target carry a variety of optical information,such as spatial information,spectral information,polarization information,propagation direction information,time information,and coherence information.The development of modern optical technology is to obtain higher-dimensional information of the target in order to reach more accurate target recognition.Hyperspectral polarization imaging technology,as one of the most cutting-edge multi-dimensional imaging technologies,can simultaneously obtain the target's twodimensional space,one-dimensional spectrum and multi-dimensional polarization information.It has important application prospects in the fields of biological microscopy imaging and can better explain the characteristic information of the biological target.This dissertation studies three spectral polarization microscopy imaging techniques to realize biological target recognition based on multi-dimensional ophthalmic information perception.The first is based on compressed sensing hyperspectral circular polarization microscopy imaging technology,which simultaneously obtains a two-dimensional space,one-dimensional circular polarization(right and left circular polarization)and one-dimensional spectral information through a snapshot measurement.The combination of the double Amish prism and the Wollaston prism makes the spectrum to expand in two orthogonal directions.Compared with the traditional compressed sensing spectral imaging technology using monochromatic dispersion elements,the spectral resolution is effectively improved.On the basis of this imaging technology,the Wollaston prism is combined,and the Wollaston prism array is used to obtain all the polarization state information at one time,forming the second imaging technology of this study——the compressed sensing hyperspectral full polarization microscopy imaging technology.Due to the different combinations of Wollaston prism arrays,two combinations of Wollaston prism arrays in the vertical and horizontal directions are discussed.This article studies the imaging and coding principles of the above two imaging methods,performs computer simulation experiments to verify and evaluates reconstruction performance,and verifies the feasibility and fidelity of the method of the aspects of image and spectral reconstruction evaluation.The third imaging technique is the hyperspectral Mueller matrix polarization microscopy imaging technique.This imaging technique can obtain the Mueller matrix of the biological target and explain the internal functional structure of the target.In this article,the hyperspectral Mueller matrix polarization microscopy imaging technology is compared with the traditional Mueller matrix polarization microscopy imaging technology,the advantages of this imaging technology are explained,and the Muller matrix of the acquired image is calculated to verify the feasibility of this imaging technology,the structure of the target image is analyzed and explained.