Bacterial Community Structure And Influencing Factors In Rhizosphere Soil Of Artemisia Ordosica And Bulk Soil In Mud Pit Coverage Area

As the country attaches great importance to the harmless treatment of drilling waste,it is important to understand the impact of mud pit solidification and landfill treatment on the ecological environment,especially the restoration and stability of the desert ecosystem.Soil microorganisms play an important role in the function and stability of ecosystems.Exploring the changes in soil and plant rhizosphere soil microbial communities affected by the solidified landfill method will provide important information for evaluating the feasibility of solidified landfill method in the desert and the restoration and stability of the ecosystem in the mud pit covered area.In this study,high-throughput sequencing technology was used to analyze the bacterial diversity,community composition,community influencing factors and co-occurrence patterns of the rhizosphere soil of Artemisia ordosica and bulk soils in 13 gas well mud pit coverage areas in the Mu Us Desert(Outside pit with natural soil as a control).The main findings are as follows:(1)The bacterial community structure of the bulk soil in the mud pit is significantly different from that of the natural soil outside the pit,while the difference in bacterial ?-diversity is small.Some bacteria involved in the carbon and nitrogen cycle are depleted from the soil inside the pit.The bacteria that are enriched in the rhizosphere soil of Artemisia ordosica for promoting plant growth,improving plant resistance and participating in the carbon and nitrogen cycle are less than those in the natural rhizosphere soil outside the pit.(2)The community structure of bacteria in different sampling soils is different.The bacterial community structure of the bulk soil is dominated by environmental factors,and the rhizosphere soil of Artemisia ordosica bacterial community structure is affected by both environmental factors and geographic factors.(3)The co-occurrence network of bacteria in different sampling soils is different.The bacterial network in the mud pit is more stable.There are many keystone species that maintain the function and stability of the ecosystem inside the pit,especially the rhizosphere soil of Artemisia ordosica inside the pit.