The Role Of Mesenchymal Stem Cells Derived From Human Embryonic Stem Cells In Angiogenesis In Vitro

Human embryonic stem cell(h ESC)is a kind of pluripotent stem cells with unlimited proliferation and multi-differentiation potential in vitro,which can be induced into a variety of cells including mesenchymal stem cell(MSC)under appropriate conditions.In addition to the function of multi-directional lineage differentiation,MSC also has a paracrine function,e.g.,screted cytokines for angiogenesis,which will benefit patients with a variety of ischemic diseases.At present,cell-based angiogenesis therapy,especially MSC therapy,has been increasingly used in the treatment of ischemic diseases.However,there are few studies on the angiogenic characteristics of h ESCderived MSC(h ESC-MSC).The present study systematically investigated into the in vitro proangiogenic effect of h ESC-MSCs which were generated in our laboratory recently,including the effect of their conditioned culture supernatants on the function of umbilical vein endothelial cells(HUVECs)and their potential to differentiate into vascular endothelial cells,in comparison with human adipose-derived mesenchymal stem cells(ADSCs)and umbilical cord mesenchymal stem cells(UC-MSCs)by cell proliferation assay(CCK-8),cell migration test(cell scratching and Transwell chamber migration),cell coculture test,living cell staining test,cell angiogenesis test in vitro,flow cytometry and fluorescence quantitative PCR.The resu1 ts showed that the conditioned medium(CM)from h ESC-MSCs cou1 d promote significantly the proliferation and migration of HUVECs,and the proliferation of HUVECs was significantly enhanced with the CM from h ESC-MSCs than that from UCMSCs;the differentiating h ESC-MSCs could form an extensive tube network in vitro after induction of differentiation towards vascular endothelial cells by all protocols tested,and the length of tubing was longer significantly than that of differentiating ADSCs and UC-MSCs,from which the tube formation appeared only occasionally.In conclusion,h ESC-MSCs can promote significantly angiogenesis in vitro,suggesting that this novel source of MSCs might be able to become an ideal therapeutic cell type for cardiovascular diseases.