Bioinformatic Gene Analysis For The Common Regulated Genes In Breast Cancer And Myocardial Infarction

BackgroundAs technological development,oncology and cardiology are becoming more and more inseparable.Breast cancer is a malignant tumor with the highest incidence among women.Cardiovascular complications related to breast cancer,especially emergencies such as myocardial infarction,are a major factor in the death of patients.Studying the relationship between breast cancer and myocardial infarction is of great significance to deepening the understanding of the disease,improving the quality of life of patients,and reducing the mortality rate of the disease.ObjectiveTo analyze the differentially expressed genes of peripheral blood lymphocyte gene chips in breast cancer and myocardial infarction by bioinformatics methods,inorder to reveal the potential relation-ship.MethodThe gene expression array data of breast cancer and myocardial infarction were downloaded from Gene Expression Omnibus(GEO),the data were pre-processed by R software to obtain differentially expressed genes."|Log Fc|?0.5,and P?0.05" were used to analyze gene expression array data,the differentially expressed genes were selected.The up-regulated genes “MI-DEGs-up,Bca-DEGs-up” and down-regulated genes “ MI-DEGsdown,Bca-DEGs-down” were analyzed by "Win Diagram",the crossover genes were selected as the common differential genes.DAVID database was used to do enrichment analysis and a protein-protein interaction(PPI)network was further constructed via STRING database.The potential relation-ship was performed using CTD database.ResultsAfter DEGs screening,112 differentially expressed genes in the myocardial infarction group(51 up-regulated genes and 61 down-regulated genes)were obtained;1502differentially expressed genes in the breast cancer group(1125 up-regulated genes and 377down-regulated genes)were obtained.Common differential genes included 11up-regulated genes(THBS1,FAM198 B,BCL6,ACSL1,SOCS3,FPR2,MAFB,CR1,CD14,CCR1 and TLR8).After GO function enrichment analysis,it was found that the common differential genes are mainly enriched in BP,involved on inflammation and complement receptor-mediated Signal pathway,magnesium ion response,negative regulation of cell matrix adhesion,My D88-dependent toll-like receptor signaling pathway,regulation of protein phosphorylation,innate immune response,cell adhesion,and I-kappa B kinase/NF-kappa B signaling.Then three hub genes were obtained through a PPI network(TLR8,CD14,CCR1).Finally,the results of CTD database analysis showed that these three hub genes are simultaneously associated with the occurrence of myocardial infarction and breast cancer.ConclusionThe three hub genes(TLR8,CD14,CCR1)are simultaneously associated with the occurrence of myocardial infarction and breast cancer.