Identification Of A Novel Group Of Bacteria And Genome Analysis Of Its Type Strain

Fristly,five strains of bacteria were isolated from the activated sludge of the sewage treatment plant of Qingdao Refinery and Chemical Co.Ltd.of Sinopec.The 16S r DNA sequence analysis showed that the similarity between the five strains was 98.73-99.93%,and they had the highest similarity with the genus Xinfangfangia of Rhodobacteraceae.Subsequently,five strains of bacteria were identified and analyzed through morphological,physiological and biochemical characteristics,chemical indicators,phylogenetic tree and genetic characteristics.The results showed that five strains of bacteria accorded with the main characteristics of Rhodobacteraceae,but differed greatly from those of standard bacteria.Five strains of bacteria clustered together with X.soil ZQBW~T in 16S r DNA phylogenetic tree to form a cluster.The results of multiphase taxonomy supported that this cluster of bacteria was a novel species of the genus Xinfangfangia of Rhodobacteraceae.DLY26~T was designated as a type strain and named X.petrolearius sp.nov.DLY26~T.Secondly,the genomic sequence of type strain DLY26~T was determined and its genomic function was annotated.Through the prediction of gene function,4,421 genes were identified.The length of coding region was 4,118,082 bp,accounting for 88.8%.Genome function annotation showed that there were 3,203 DLY26~T proteins with definite biological functions,and the homologous proteins of DLY26~T were from 356 species,of which the highest homology was Rhodobacter sp.SW2,accounting for 19.5%.Finally,by analyzing the metabolic pathway of type strain DLY26~T,it was found that there were novel nitrile hydrolases in the metabolic pathway of aminobenzoate degradation in the strain.Therefore,the growth of DLY26~T in nitrile compounds was preliminarily measured in this paper.It was found that DLY26~T could degrade many nitrides,among which the degradation effect of acrylonitrile was better,and the degradation rate could reach 79.4%.Metabolic pathway analysis showed that DLY26~T contained citric acid circulation pathway,iron metabolism pathway and amino acid salt degradation metabolic pathway,among which the amino acid salt degradation metabolic pathway contains nitrile hydrolase.Through phylogenetic analysis,it is found that two nitrile hydrolase in DLY26~T are clustered independently and have high novelty.The growth of DLY26~T in nitrile compounds was preliminarily determined,and it was found that it could degrade a variety of nitrile objects,among which the degradation effect of acrylonitrile was better,and the degradation rate could reach 79.4%.These results laid a foundation for further exploring such nitrile hydrolases in the future.