The Biotransformation Of Arsenic In Marine Fish And Mice And Its Ecological And Health Risk Indication

Arsenic is a highly toxic chemical substance,which will pose serious threat to the health of human body and other organisms in the environment,and there are huge ecological risks / environmental hazards.The toxicity of arsenic is closely related to its existing speciation.The most common highly toxic arsenic in the environment is inorganic arsenic with trivalent [arsenite,As(?)] and pentavalent [arsenate,As(V)],while organic arsenicals,monomethylarsonic acid(MMA)and dimethylarsinic acid(DMA)are less toxic.trimethylarsine acid(TMAO),arsenobetaine(As B),arsenocholine(As C)are considered to be slightly toxic or non-toxic.Arsenic is ubiquitous in the marine environment.Seafood usually contains high concentration of arsenic.Meanwhile,seafood accounts for 90% of all arsenic in human diet.Therefore,high arsenic concentration in seafood can cause potential hazards to biological and human health.The purpose of this paper is to explore the possible health effects of arsenic exposure in marine fish and whether the high concentration of arsenic in fish will damage human health,so as to provide scientific basis for the protection of marine ecological environment and the maintenance of marine food safety.1.The calculated concentrations were 100 ?g/g inorganic arsenic As(V)and organic arsenobetaine(As B)are exposed to the marine fish(pearl gentian grouper)respectively for 14 days and purified for 20 days,the samples were taken regularly and the arsenic in blood,organs and tissues of fish were analyzed qualitatively.The results show that:(1)There was no systemic toxicity of As(V)and As B to the fish after 14 days of continuous exposure,which did not affect the normal growth of fish;(2)The fish could rapidly excrete toxic inorganic arsenic As(V),while non-toxic organic arsenic As B tended to accumulate in its muscle;(3)The fish could convert the As(V)into low toxicity MMA and DMA,and even non-toxic As B,which was stored in the muscle of fish,this process of biotransformation can reduce the harm of inorganic arsenic to the health of organisms,and reduce the ecological risk of inorganic arsenic.2.In view of the high concentration of arsenic in marine fish,we gavage mice with five species of arsenic: As(?),As(V),MMA,DMA and As B at 1 mg/kg body weight for 14 days,and take the internal organs and muscle tissues of mice for qualitative and quantitative analysis of arsenic.The results showed that:(1)five species arsenic of1mg/kg had no toxicity to mice,and the weight of mice tended to be stable during exposure;(2)The accumulation of different speciation of arsenic in organs and tissues of mice showed arsenic species-dependence,mainly manifested as relatively high accumulation of total arsenic in almost all major internal organs including the stomach,intestine,kidneys,liver,lung,spleen,heart and muscle in stomach,intestine,heart,brain and muscle of i As [especially As(III)] exposure,the highest accumulation of MMA in kidney,while low accumulation in other organs,The accumulation of DMA was low in all organs and the highest accumulation of As B was in liver,lung and spleen;(3)Organic arsenic MMA,DMA and As B were significantly transformed into more toxic inorganic arsenic [mainly as As(V)] in mice,and the degradation of organic arsenic increased the risk of organic arsenic intake to human health.3.This study shows that the biological transformation of toxic inorganic arsenic into non-toxic organic arsenic by marine fish can reduce the ecological health risk of arsenic.However,the degradation of organic arsenic into inorganic arsenic by mammals increases the health risk of arsenic to organisms.Therefore,we appeal to more researchers to pay attention to the risk assessment of human long-term intake of organic arsenic from seafood.