Preliminary Study On The Molecular Mechanism Of Flight Muscle Degradation In The Domestic Cricket Acheta Domesticus

Flight muscle degradation is a common phenomenon in the long-term evolution of insects,and it is widely found in insect species with "flying ability".Flight muscle degradation is a survival adaptation of insects to the external environment,which can effectively conserve and utilize the limited substances and energy in the body.For a long time,this developmental phenomenon has always been a hot research issue for scholars at home and abroad,exploring the molecular mechanism of flight muscle degradation is the theoretical basis to interpret the growth and development of insects.At present,the phenomenon have been a few studies in Coleoptera and Hemiptera such as Lissorhoptrus oryzophilus and Sitobion avenae,but there is still no conclusion in Orthoptera,especially the less related molecular mechanism of Gryllidae.Domestic cricket(Acheta domesticus)is a common group in the Orthoptera Gryllidae,it is widely distributed and easy to feed,it is an ideal experimental insect in the study of flight muscle degradation.In this study,through transcriptomics,proteomics,biochemistry analysis and RNAi and other technical means,a preliminary study of the molecular regulation mechanism of the degradation of the flight muscle of domestic crickets was carried out.The conclusion are as follows:1.The degradation of the flight muscle of domestic crickets have regularity.The domestic cricket is in its early stage of emergence,the flight muscles of female and male worms are basically fully developed,they are full and full in shape.After the emergence of adult domestic crickets,some domestic crickets begin to degrade their flight muscles.In the experimental conditions of this study,9-11 days after the emergence of domestic crickets,most of the individual flight muscles of domestic crickets in the population degraded,and the degradation regularity of flight muscle of female and male domestic crickets was basically the same,and there was no significant difference.2.Fatty acid binding protein is involved in regulating the flight muscle degradation of domestic crickets.(1)Taking the 1685 DEGs obtained from the transcriptome data and 1666 DEPs obtained from the proteome data of two periods before and after the degradation of the flight muscle of domestic cricket as the data set,the transcriptome and proteome sequencing results were comprehensively analyzed.Select 3 differentially expressed genes related to flight muscle degradation as candidate genes: Adom Fatty acid binding protein,Adom Troponin T,Adom Actin.(2)RNA interference candidate genes(Adom FABP,Adom Troponin T?Adom Actin),and statistically analyzed the degradation of the flight muscles of domestic crickets in different injection groups,it was found that on the 6th day after ds RNA injection,the degradation of flight muscle was significant in the ds Adom FABP group,compared with the wild-type control group and the ds GFP group.Indicating that Adom FABP gene participates in the regulation of flight muscle degradation of domestic cricket.Proteomic sequencing results showed that Adom FABP protein was expressed in the flight muscles of domestic crickets,and the expression level after flight muscle degradation was lower than that before flight muscle degradation,the results of proteomics sequencing further proved that Adom FABP is involved in regulating the degradation of flight muscle of domestic crickets.In summary,this article takes domestic crickets with the phenomenon of "flying muscle degradation" as the experimental object,with the help of molecular experimental methods such as transcriptomics,proteomics,biometric analysis and RNAi,it is found that Adom FABP gene is involved in the molecular regulation of flight muscle degradation of domestic crickets.it provides a theoretical reference for revealing the molecular mechanism of flight muscle degradation in Orthoptera insects.