Soil Improvement Of Reed Biochar On Typical Green Land In Xiong'an New Area

As an environmentally friendly material,biochar plays important roles in improving soil physical and chemical properties and enhancing soil microbial activities.This study mainly focused on the reeds in Baiyangdian Lake(BYD)to explore the impact of the application of the biochar on soil bacterial and fungal communities.The most suitable pyrolysis temperature is used to prepare reed biochar,and used to improve the Xiong'an New Area's green soil;The soil p H,water content,electrical conductivity,nitrate-nitrogen,and nitrate nitrogen were measured after addition of 1% reed biochar to the soil for 36 days,and ammonium nitrogen and other indicators were also used to evaluate the impact of reed biochar on soil physical and chemical properties;through high-throughput DNA sequencing,the community composition structure and diversity of bacterial and fungal taxa as well as soil physical and chemical properties in soil samples were analyzed before and after biochar application.The main research results obtained from the experiment are as follows:1.With the increase of pyrolysis temperature,the average pore diameter and H/C of biochar decrease.The average pore diameter of biochar prepared at 300 ? without post-treatment was 13.9 times that of 800 ? pyrolysis temperature.The obtained biochar of300 ? pyrolysis temperature had the highest char yield,N and H content,so the biochar prepared at 300 ? pyrolysis temperature was selected as the green soil amendment;2.In the rhizosphere soil of white wax,lupine,and Juniper,the p H value of the soil decreased and tended to be neutral after adding biochar for 36 days;the soil electrical conductivity was significantly increased compared with the control group,which were 124%,133%,and 126%,respectively;Soil water retention capacity has been improved,but it has not reached a significant level;when compared with uncultivated soil,the application of biochar increased the total carbon content in the rhizosphere soil of lupine and white wax,but there was no significant effect in the rhizosphere soil of Juniper;The total nitrogen content in the rhizosphere soil of Juniper increased significantly,and the total nitrogen content in the rhizosphere soil of Luan tree and white wax did not change significantly;the content of nitrate nitrogen and ammonium nitrogen increased in all soils,but did not reach significant level.3.In the tested soil,the bacteria were dominated by Actinobacteria,Proteobacteria,Acidobacteria and Chloroflexus.Dominated fungal phyla were Ascomycota and Mortierella.After applying biochar for 36 days,the composition of the bacterial and fungal communities in the three tree species' rhizosphere soil changed.The relative abundance of Acidobacteria and Chloroflexum in the soil bacterial community decreased,and the relative abundance of Firmicutes increased.Alpha diversity results showed that the bacterial Chao1 index,ACE index and Shannon index increased in the rhizosphere soil of the three tree species,indicating that the bacterial community richness and diversity increased,while the fungal Shannon index decreased in the rhizosphere soil,which affected the community richness.There were no significant changes in the fungal Chao1 index and Shannon index in the rhizosphere soil of Koelreturea paniculata and Sabina chinensis.The water content,electrical conductivity,nitrate-nitrogen,organic carbon,and total nitrogen in the physical and chemical properties of the soil would affect the soil microorganisms.The abundance of bacterial phyla like Bacteroides and Chloroflexum and fungal phyla such as Chytridomycetes and Globococcus were closely related to changes in soil properties.