Study On Key Adhesion Genes And Their Selective Adhesion Behavior Of Mytilus Mussel

Mussels,a kind of marine fouling organisms with strong adhesion,can adhere to almost any surface in the ocean,such as ships,oil platforms,fishing equipment and other artificial facilities.This will not only cause huge economic losses to the society,but also cause biological invasion and destroy the ecological balance.Inhibiting the expression of adhesive protein in mussel is the key link of inhibiting its adhesion,and is also a scientific problem in the field of pollution prevention.Therefore,it is of great significance to study the adhesion gene and its selective adhesion behavior of mussels.Based on the analysis of the tissue structure related to the adhesion of Mytilus edulis,this paper focuses on the selective adhesion of Mytilus edulis.Through biological microscope and scanning electron microscope(SEM)analysis the morphology of Mytilus edulis adhesion related organization structure,by real-time fluorescent quantitative PCR(RT-q PCR),transcriptome sequencing technology research such as purple foot mussel adhesive proteins in the specificity of the related regulation of gene expression,also by common Mytilus edulis adhesion experiments to explore its selective adhesion to different materials.In order to understand the adhesion structure of the Mytilus edulis,this paper dissected the Mytilus edulis,observed and analyzed the tissue structure related to its adhesion,and found that the elastic proximal end and rigid distal end of the filament as well as the larger plaque interface can provide the Mytilus edulis with the ability to resist impact.The position of glands in the foot and the ciliary structure outside the foot contribute to the adhesion process of the mussel to the material.RT-q PCR showed that the glands in the distal depression of the foot were the sites for the secretion and synthesis of adhesive proteins.In this study,transcriptome sequencing technology was used to analyze the differential genes in the distal end of the foot of Mytilus edulis with different secretion states,and 210 differential genes were found between the two groups: 93 up-regulated genes and 117down-regulated genes.By comparing the differential genes with the database,it was found that they were mainly involved in the metabolic processes such as signal transduction,amino acid transport and metabolism.Through the enrichment analysis of differential genes,combined with the directed acyclic map of molecular function,it was found that the differential genes were enriched in the metabolism of indoles and aromatic amino acids,which indicated that these substances were involved in the secretion and modification of adhesive proteins.In this study,the selective adhesion behavior of Mytilus edulis on KH-550,KH-560,KH-570,glass,epoxy resin(E44),polyurethane and polydimethylsiloxane(PDMS)were studied by selective adhesion experiment,and the expression differences of key adhesion proteins regulatory genes were studied by RT-q PCR.Through the related experiments on the adhesive proteins secreted by Mytilus edulis on different materials,it was found that Mytilus edulis secreted proteins with different contents of dopamine on different materials,and these proteins had different adhesion on different materials.It was found that the expression level of the related genes of adhesion proteins in the 5 kinds of materials was different through the experiments of the foot tissues adhured to different materials.Through the experiment of adhesion selection,it was found that the Mytilus edulis was more inclined to adhere to the surface of the material with low roughness.