Device Based On Ultrasonic Biological Effect And The Preliminary Study On The Early Development And Application Of Animals And Plants

With the continuous upgrading and improvement of ultrasound engineering and biomedical related technologies,ultrasound technology has gradually entered various industries of biomedical engineering and achieved satisfactory results.The stimulation mechanism and influence of ultrasound on cells have been continuously discussed and studied by researchers.Ultrasound is widely present in the living environment.As a physical factor,its biological effects have always attracted people's attention.The mechanism of ultrasound on cells has been constantly understood by people.For example,low-intensity pulsed ultrasound can speed up material transmission by changing cell permeability,accelerate cell growth,proliferation,or directional differentiation,and use ultrasound thermal effects to kill cancerous cells.The stimulating effect of ultrasound on cells is related to factors such as ultrasound frequency,power,sound intensity,and duration of action.Appropriate power or sound intensity is conducive to the expression of related proteases in cells to accelerate cell proliferation or promote differentiation,but the stimulation time is too long or power too high will cause damage to cells,which is not conducive to cell growth.In addition,an appropriate duty cycle also has a great impact on cell proliferation,so it is very urgent to carry out research on the selection of ultrasound parameters and the theory of cell stimulation.Based on the biological effects and mechanism of ultrasound on cells,this paper designs an ultrasonic cell stimulation device and uses the device to study the effects of ultrasound on the early development of animals and plants.The main research content of this paper includes the following aspects:(1)Through the theoretical analysis of spherical cells,ellipsoidal cells and rectangular cells,the characteristic frequencies of zebrafish embryo cells and mung bean seed cells are obtained using COMSOL Multiphysics 5.4 finite element simulation software,and based on this frequency,a system capable of Applied to general biological laboratories,it can realize the ultrasonic cell growth stimulation device that can adjust parameters such as different frequency,power,action time and duty cycle.The device mainly includes a display unit,a transducer drive unit,a temperature control detection unit,a circulating water control unit,and a central control unit.After completing the design and manufacture of the device,the performance test and functional verification of the device were carried out with the help of animal and plant cell stimulation experiments.(2)Animal experiment--Based on this device,the effect of ultrasound on the growth and development of zebrafish embryonic cells is studied.Designed an orthogonal experiment plan,carried out a three-factor and three-level study on the ultrasonic stimulation frequency,stimulation time and signal excitation voltage to investigate the impact of the ultrasonic device on the hatching speed of zebrafish eggs,and finally determined the optimal plan: Under ultrasound conditions with a frequency of 1.26 MHz,a stimulation time of 30 min,and a signal excitation voltage of 3Vpp,the egg hatching index is significantly higher than that of the control group,which proves that ultrasound stimulation under appropriate conditions can promote embryonic development of zebrafish.(3)Plant experiment--Based on this device,the effect of ultrasound on the growth and development of mung bean seeds was studied.The device is used to study the effects of ultrasonic frequency,stimulation time,and sine wave signal excitation voltage on the germination index of mung bean seeds.On this basis,different waveforms(continuous wave and pulse wave)ultrasound and ultrasound synergy with different concentrations of sodium chloride are studied.The effect on the germination of mung bean.The experimental results show that pulse wave ultrasound is better than continuous wave ultrasound.When the pulse wave duty cycle is 20%,the mung bean germination rate is the highest under the conditions of 48 k Hz,35 min,and 10 Vpp.This frequency is basically consistent with the results of simulation calculation,and the volume The Na Cl solution with a molar concentration of 17.09mmol/L and ultrasonic treatment can greatly accelerate the germination of mung bean,and the germination rate is 41% higher than that of the control group.