The Synergistic Effect Of Phosphorus And Microorganisms On Rice Growth And Bacterial Community Structure Diversity With Different Root Systems

Rhizosphere microorganisms are the general term for the microorganisms that are enriched in the root system of plants under the influence of biological and non-biological environmental factors.They are affected by various factors such as soil type,physical and chemical properties,and nutrient elements,and have the functions of resisting pests and diseases and promoting plant growth and development.Phosphorus is one of the important elements for the normal growth and metabolism of plants.It is easily adsorbed and fixed in the soil,and cannot be directly absorbed and utilized by plants.It becomes the main limiting factor for plant growth.The rhizosphere effect has become an important mechanism for regulating the global carbon(C)and nitrogen(N)cycle,but the role of root morphology in regulating the rhizosphere effect is still unclear.In recent years,with the development of molecular biology,the rise of rhizosphere microbiome research has provided brand-new technologies and ideas for improving the absorption and utilization of phosphorus by plants.This project uses different root morphology rice varieties as experimental materials,uses high-throughput sequencing methods,and combines soil phosphorus components and rice biological indicators to explore the growth of rice with different root morphology under the synergistic effect of phosphorus and microorganisms,as well as rhizosphere and root bacteria The microbial community structure and diversity are expected to reveal the root-phosphorusmicrobe interaction mechanism and provide a theoretical basis for the development and excavation of new plants to promote phosphorus absorption and high-efficiency microorganisms.The main results obtained are as follows:(1)Compared with the wild-type,the short-root mutant's biomass and plant height were significantly lower than the wild-type under the synergistic effect of phosphorus and microorganisms.In the rhizosphere,the phosphatase activity of short-root mutants was significantly correlated with microbial diversity.In the root system,the accumulation of phosphorus in the shoots of short-root mutants was significantly correlated with bacterial diversity.Under the combined influence of phosphorus,microbial communities and plant roots,soil microorganisms gathered in the rhizosphere of short-root mutants to form specific microbial communities,among which the abundance of Enterobacter and Exiguobacterium were significantly higher than The wild-type,Clostridium spp.has a significantly lower abundance.(2)Compared with the wild-type,the root hairless mutant has no significant effect on the growth and development of rice under the combined action of phosphorus and microorganisms.The bacterial community diversity index of the rootless mutants was significantly higher than that of the wild type,which was related to phosphatase activity,soil available phosphorus content,and phosphorus accumulation in the shoots of plants.Under the combined influence of phosphorus,microbial communities and plant roots,the rhizosphere of the rhizosphere of the rhizosphere-less mutants has more phosphorus solubilizing bacteria than the wild type in the low phosphorus environment,such as Pantoea and Enterobacter,which promotes phosphorus absorption.(3)Compared with the wild type,the non-lateral root mutant has significantly lower plant height and biomass than the wild type under the synergistic effect of phosphorus and microorganisms.There is a significant difference between the non-lateral root mutant and the wild-type rice microbiome.The wildtype has a higher species diversity.Under the combined action of different phosphorus and microorganisms,the different bacterial genera are mainly concentrated in the phylum Actinobacteria and Firmicutes.In summary,the results of this study show that the length of the root system plays an important role in the growth and development of rice,followed by lateral roots,and root hairs have the least effect on the growth and development of rice.The effect of microorganisms on rice with different root morphologies indicates that the worse the root function,the greater the effect of microorganisms.Rice with.different root morphology has different microbial community diversity and structure under the syner.gistic effect of phosphorus and microorganisms.