Comparative Analysis Of Different Sources Of Lactobacillus Plantarum Genome And Carbohydrate Utilization

This experiment studies the gene specificity of L.plantarum from different sources and its genetic diversity in different environments,and explores the similarities and differences in the adaptability of two strains of L.plantarum A8 and L.plantarum P9 in the intestinal habitat and plant surface.Use genomics to compare the predicted carbohydrate active enzymes(CAZymes)related genes,and explore the characteristics of carbohydrate active enzymes between the two strains through phenotypic tests,so as to provide a theoretical basis for the development of characteristic strains.This study analyzed the genomes of L.plantarum A8 and L.plantarum P9 isolated from animal intestines and plant surfaces,using a combination of Next Generation Sequencing(NGS)and third-generation single-molecule sequencing technology,based on The Pac Bio Sequel sequencing platform sequenced L.plantarum A8 and L.plantarum P9.Carbohydrate-active enzymes(CAZy),Koyto encyclopedia of genes and genomes(KEGG),and Clusters of orthologous genes(COG)databases are used to annotate the genome functions;CGView software is used to draw the genome circle map of the strain.Use comparative genomics to compare and analyze the genomes of L.plantarum from other habitats that have been published.And use sugar fermentation experiment and real-time fluorescent quantitative PCR experiment to verify the expression of genes involved in carbohydrate synthesis and metabolism in two strains of L.plantarum.The results of the study showed that the genome sizes of L.plantarum A8 and L.plantarum P9 were different.By constructing a phylogenetic tree,it was found that the two strains belonged to the same branch with L.plantarum from other sources,and that L.plantarum P9 was the same as L.plantarum from breast milk.The L.plantarum WLPL04 strain is the closest,while the L.plantarum A8 and L.paraplantarum DSM10667 are the closest.Through gene family analysis,the two strains share2643 genes,including common heat shock protein,phage shock protein C,and some cold shock proteins.There are 321 and 336 unique genes in L.plantarum A8 and P9,respectively.The unique genes in L.plantarum A8 are mainly involved in DNA replication,ABC transfer system(ABC transfer system),PTS system(phosphotransferase system),sulfonate transfer system,and amino acid Metabolic pathways such as biosynthesis;and the unique genes of L.plantarum P9 are mostly involved in carbohydrate transport and metabolism,such as rpi A gene,lac Z gene,Fru A gene and so on.COG annotation results show that the functions of the two strains are concentrated in carbohydrate transport and metabolism,transcription,amino acid transport and metabolism,etc.Through KEGG annotation,it is found that the metabolic pathways of the two strains are mainly concentrated in genetic information processing,signal transduction,and cellular processes.Metabolic pathways such as carbohydrate metabolism.CAZymes annotation results showed that the carbohydrate enzymes of the two strains were concentrated in glycoside hydrolase,glycosyltransferase,carbohydrate binding module families.The sugar fermentation test shows that there is a difference between the two in the utilization of arabinose.And the expression levels of P9 carbohydrate enzyme genes(glf,nan K,iol G,hxl B,HEXA?B,rha D)were significantly higher than that of A8(P<0.01).(Conclusion)Both A8 and P9 are rich in carbohydrate active enzymes,and the carbohydrate utilization capacity of P9 is higher than that of strain A8.The genome information of L.plantarum was analyzed by comparative genomics methods,and L.plantarum attached to the surface of animal intestines and plants was found.The genetic characteristics of the strains are different.L.plantarum P9 has good carbohydrate utilization ability.Among them,the L-arabinose isomerase activity is relatively strong,and it can be bio-converted into D-tagatose in industry,which is widely used in diet food.Diabetes products.Therefore,in-depth research on the future application potential and application direction of the two L.plantarum strains will provide a scientific basis for further development and utilization.