Effect Of Sulfate Reduction Pathway Inhibitor Enrichment Culture On The Community Structure Of Microorganisms

Sulfate-reducing bacteria(SRB)is an anaerobic microorganism that uses sulfate as a terminal electron acceptor.It is widely distributed in marine sediments and participates in the recycling process of various substances,such as the degradation of organic compounds,the global sulfur cycle,and the carbon Circulation and nitrogen circulation and other processes.At present,most studies focus on the community,diversity,metabolism,activity and ecological effects of SRB,but few studies report the potential role and contribution of SRB groups to the construction of bacterial communities in the process of anaerobic degradation of macromolecular substances.In the early stage of our laboratory,a perfect marine sediment microbial enrichment culture system was established,and during the enrichment culture process,it was found that the abundance of SRB groups increased significantly.This system can be used as a microcosm to study the anaerobic degradation of macromolecules in SRB groups.The role of the process in the process of assembling the community structureThis experiment uses the microcosm system of enrichment culture,suppresses the SRB group by adding sulfate reduction pathway inhibitor sodium molybdate,and analyzes the enrichment by combining 16S rRNA gene amplicon high-throughput sequence analysis and changes in physical and chemical factors.During the process,when the SRB group is inhibited,the molecular ecological network of the bacterial community and the changes in the cultivable bacterial community.In order to obtain the influence of SRB groups on the changes of microbial community structure in enrichment culture,it provides a certain reference plan for studying the function and role of SRB groups in the community structure,and also provides ideas for later functional research on a specific group.The main research results are as follows:1.Using high-throughput sequence analysis of 16S rRNA gene amplicons,it was found that sodium molybdate can significantly inhibit the relative abundance of sulfate-reducing bacteria groups during the enrichment culture process,and affect other microbial groups,making the dominant groups from the original Desulfobacterota and Synergistota transformed into Fusobacteriota,Firmicutes.2.Community molecular network analysis showed that the addition of sodium molybdate in the enrichment culture process can increase the complexity of the community structure and the competition between communities.After 30 days of enrichment,the bacterial community structure in this treatment group is more likely to collapse,indicating that sulfate reducing bacteria.The community has a greater effect on maintaining the stability of the microbial community structure during the enrichment process.3.The analysis of physical and chemical factors in the enrichment culture process found that the addition of sodium molybdate can change the correlation between the difference in microbial community structure and Fe2+?SO42-and SO32-.4.In the isolation of cultivable bacteria,307 strains of bacteria were isolated and cultured from the enrichment culture system.Among them,the experimental group and the control group with sodium molybdate added had significant differences in species level diversity.