Preparation And Activity Research Of Bone Collagen Peptides From Xiang Hai's Anser Cygnoides

Collagen is an important part of the organic matrix of bone.Its hydrolysate is collagen peptide,which has a variety of physiological activities,such as anti-oxidation,anti-aging and anti-osteoporosis.In this paper,goose bone is used as the raw material for preparation of collagen peptides.First of all,the preparation technology of geese collagen polypeptide was investigated,and then the antioxidant properties and osteoblast proliferation and differentiation activity of geese collagen polypeptide were explored.We hope that these findings will provide technical parameter guidance for the research and development of geese and related products.The experimental results are as follows:(1)We analyzed the basic components of the goose bone,in which the protein content was as high as 48.12%.We used the degree of hydrolysis as an indicator to determine that the papain,neutral protease and alkaline protease constituted the compound enzyme used in the experiment,and determined that its mass ratio was 1:2:1.Through single factor experiments and response surface experiments to study and optimize the enzymatic hydrolysis process,the optimal enzymatic hydrolysis process parameters are:under the conditions of 50.4?,p H 7.1 and 6100 U/g,the best hydrolysis degree was26.34%.Enzymatic hydrolysis of anser cygnoides bone to prepare collagen peptides under optimal conditions,the molecular weight of collagen polypeptide obtained by enzymolysis was determined by SDS-PAGE electrophoresis and molecular exclusion chromatography,the molecular weight range measured by this method is 851?11092 Da.The ultra-filtration membrane method was used to classify and purify the geese collagen peptides prepared under optimal conditions,and three peptide components BCP1,BCP2,and BCP3 with molecular weights of 5 to 10 k Da,3 to 5 k Da,and less than 3 k Da were obtained,and the three components account for 97.82%of the total mass of the peptide.Through the analysis of the amino acids of the collagen polypeptide,it was found that the content of glycine is up to 27.7%,and proline,hydroxyproline and alanine are also abundant.(2)It was found that the geese collagen polypeptide and its separated components showed strong antioxidant properties,and the fractionated polypeptide components had stronger antioxidant capacity by measuring the reducing power of geese collagen peptides and their separated components,as well as the DPPH radical scavenging rate,superoxide anion radical scavenging rate,and hydroxyl radical scavenging rate.Under the same conditions,the free radical scavenging rate and reducing power of each component were BCP3>BCP2>BCP1>BCP.When the mass concentration of the BCP3 component of the goose bone collagen peptide was 12 mg/m L,the reducing power A_700nmvalue was 0.915,and the clearance rates of DPPH radical,superoxide anion radical,and hydroxyl radical were 89.23%,90.67%,88.17%,respectively.(3)Based on the MC3T3-E1 cell proliferation and alkaline phosphatase activity measurements,it was found that the collagen peptides of geese bone and the components of each polypeptide can promote the proliferation and differentiation of osteoblasts.When the concentration of administration is 100?g/m L,the effect of each component on the proliferation and differentiation of osteoblasts is extremely significant,and the BCP3polypeptide component has the strongest promotion activity.When administered at 100?g/m L for 3 days,the osteoblast proliferation rate is 57.14%;And when the cells are cultured for 7 days,ALP activity increased by 15.90%.