Study On The Secondary Metabolites Of Two Marine-derived Microorganisms

Marine micro-organisms are very rich in secondary metabolites and,because of the specificity of the marine environment,the specificity of their secondary metabolites and the diversity of their metabolic pathways are important sources of bioactive compounds.This experiment was based on Actinobacteria SOA-026 and Fungi Bzo-L062-2 which were earlier collected by the group from sources in the South China Sea,and they were studied by I6 s r RNA and ITS r RNA sequence analysis.The SOA-026 was identified as Streptomyces sp.and Bzo-L062-2 was identified as Myrothecium sp..The ethyl acetate metabolites of Actinobacteria SOA-026 and Fungi Bzo-L062-2 were extracted and separated by normal-phase silica column chromatography?Sephadex LH-20?HPLC and other methods.Eight compounds were isolated and identified from the fermentation products of Actinobacteria SOA-026.According to the analysis of physicochemical properties and modern wave spectroscopic characteristics,eight compounds were obtained.Identified as cyclo-(pro-val)dipeptide(1),Cyclo-(leucyl-4-hydroxy-prolylleucyl-4-hydroxy-proline)(2),cyclo-(tyro-pro)dipeptide(3),cyclo-(chromatic-prolyl)dipeptide(4),thymine deoxyribonucleoside(5),methyl p-hydroxyphenylacetate(6),methyl p-hydroxyphenylacetate(7),4?,5,7-trihydroxyisoflavone(8).Nine compounds were isolated and identified from the fermentation products of Fungi Bzo-L062-2,and also Identified as(-)-(S)-(3,5-dihydroxy-4-methylphenyl)-(tetrahydro-2-furanyl)methaon(9)?(+)-(R)-(3,5-dihydroxy-4-methyl-phenyl)-(tetrahydro-2-furanyl)methaone(10)?Methyl 4,6-dihydroxy-5-methylbenzoate(11)?(+)-S-(3,5-dihydroxy-4-methylphenyl)-(2-methoxytetrahydro-2-furanyl)methaone(12)?(-)-R-(3,5-dihydroxy-4-methylphenyl)-(2-methoxytetrahydro-2-furanyl)methaone(13)?lunatinin(14)?monomethylsulochrin(15)?(7S,8S)-7-hydroxy-3-(3-hydroxypropyl)-4-methoxy-7-methyl-6-oxo-7,8-dihydro-6Hisochromen-8-yl butyrate(16)?butyrolactone ?(17).Of the 17 compounds obtained from the above isolation and identification,compounds 9,10,12,13,and 16 are new compounds not reported in the literature.Compounds 9,10,and 12,13 are enantiomers obtained after chiral splitting.Some of the compounds that have been isolated and identified were tested for nitric oxide(NO)-producing inhibitory activity,and Compounds 9,10 at concentrations that are not cytotoxic show better performance against multiple macrophages(RAW 264.7)NO produces inhibitory activity.The antimicrobial activity of the compound is also being tested.