The Design Of A Visualized Cell-electrofusion Device

As an important means of cell engineering,cell fusion technology provides powerful support for gene regulation,cell immunity,gene positioning and other studies,and has high application value in many fields.As one of the physical induction methods,cell electrofusion has wide applicability,little cell damage,and no residual toxicity,which makes it gradually become the mainstream method of cell fusion.In the process of conducting cell electrical fusion experiments,in order to further study the mechanism of cell fusion and evaluate the effects of cell fusion experiments,researchers often need to observe and record the process of cell fusion.Traditional commercial cell electrofusion instruments use parallel plate electrodes and corresponding fusion chambers to complete cell fusion operations,which is difficult to observe during the experiment.With the application of cell electrofusion chips based on MEMS technology,researchers can use an optical microscope to observe and record the cell fusion process.However,the optical microscope is bulky and difficult to move,and this requires multiple devices to be operated.The steps are cumbersome and may cause contamination of the cells.At the same time,the problem of the small field of view of the optical microscope will also affect the experimental observation.The lensless digital holographic micro-imaging technology emerging in recent years has the characteristics of simple structure,large observation field,high integration and low cost.Many researchers have used this technology to replace optical microscopes and designed a variety of low-cost and miniaturized biological cell observation and classification platforms,which have achieved good results.Based on this,this paper puts forward a visualized design of cell electrofusion device based on the research of cell electrofusion device and lensless digital holographic imaging technology.Through analysis of the principles of cell electrofusion and lensless digital holography,combined with existing cell electrofusion instruments,the specific requirements and corresponding design indicators of the device are proposed.According to the proposed design indicators,the selection of core devices is completed,and the corresponding functional circuits are designed and built.Afterwards,each functional module is tested to verify whether the device meets the design index.The experimental results show that the device can generate a signal that meets the design index and complete the queuing experiment.The frequency of the cell queuing signal can be up to3 MHz,and the peak-to-peak value can reach 24 V.The pulse amplitude can reach 40 V,the pulse rise time is less than 25 ns,and the load capacity is good.And combined with the lensless digital holographic imaging module,the device can observe objects on a cell scale in real time,with a resolution of up to 4 ?m.