Novel DNA Purification Strategy Via The Removal Of Protein By MoS₂

As the carrier of genetic information,DNA is the material basis of gene expression and plays an important role in biological activities such as normal growth,development and reproduction of organisms.The extraction/purification of DNA is an important task in life science research,and the employment of suitable extraction/purification method is the key to achieve purified DNA sample.In present study,a rapid and efficient extraction/purification protocol is developed for the purification of DNA by using MoS2 particles as solid-phase adsorption medium,based on the adsorption and removal of protein by MoS2 particlesFirstly,MoS2 particles are prepared via ultrasonic-stripping method.The obtained MoS2 particles are characterized by the technique of scanning electron microscopy,atomic force microscopy and zeta potential analysis.The adsorption performances of protein and DNA onto MoS2 particles are investigated.Under pH 5.0,complete adsorption of proteins in 1.0 mL solution(1 mg mL-1)can be readily achieved by 5.0 mg MoS2 particles,while nearly no adsorption of DNA take place under the same condition.The adsorption equilibrium of proteins onto MoS2 particles is reached at a adsorption time of 20 min,and the adsorption behaviors of proteins onto MoS2 particles fit Langmuir model,corresponding to an adsorption capacity of 58.82 mg g-1.Base on the adsorption difference of MoS2 particles towards DNA and proteins,a novel strategy is thus developed for the separation/purification of DNA from complicate matrices.The elution manipulations,which are usually needed in conventional solid-phase extraction protocols for DNA purification,are avoided in this adsorption-and-removal-of-protein-based strategy.Therefore,the solvent effect on DNA structure is eliminated as no elute or chaotropic agent are added in the DNA purification process.This proposed strategy has been applied to the separation/purification of DNA from a series of real samples,including synthetic ?-DNA sample,chicken whole blood and E.coli cell lysate.The successful PCR amplification of 500 bp ?-DNA fragment,200 bp chicken whole blood gene fragment and 150 bp E.coli gene fragment are achieved by using the purified DNA samples as PCR templates.These results well suggest that the DNA samples purified with the adsorption-and-removal-of-protein-based strategy based on are of high purity,meeting the purity requirements of ensuing DNA assays.