Research On Cell Stiffness Characteristics Based On Mechanical Modeling

As the most basic unit of all living organisms,the study of cell mechanical properties has received extensive attention.Different types of cells respond differently to the same external stimulus.Certain types of cells also respond differently to external stimuli when they are in a normal or diseased state.Cells of different structural or mechanical properties display unique characteristics when performing respective physiological functions.Therefore,it is an innovative development to reconstruct complex cell structures through computer simulation software,to study and analyze the mechanical properties of cells,to reasonably predict changes of cells under external forces,and to provide theoretical basis for the practical exploration of life sciences by engineering research.This article takes mouse embryo osteoblasts MC3T3-E1 as the research object and mainly studies the following aspects.On the basis of in-depth analysis of the theoretical issues involved in the process of building the cell model,combining with the specific performance parameters of each component in the cell protein fiber grid system,the theoretical equation of the cell model was obtained by theoretical calculation,and the boundary conditions and load constraints required for the simulation analysis were determined.Based on the actual performance parameters of each component in the cell protein fiber grid system,the simulation analysis software,MATLAB,was used to analyze the stiffness characteristics of the cell plasma membrane,to clarify the scale of the flat cell model,to generate the internal components of the model micro-unit,and to determine the internal structure of the model.Through the simulation,the change trend of the elastic modulus of the cell plasma membrane under different conditions was analyzed from the aspects of concentration of the bound protein,the initial tensile force,the length and concentration of the actin filament,and the radian and concentration of the actin filament binding bundle.The influence of various factors on the stiffness characteristics of the cell plasma membrane was studied.The constructed cell model was extended from a flat state to a three-dimensional state.The influence of the stiffness characteristics of the plasma membrane on the length and concentration of actin filaments,and the radian and concentration changes of actin filament bundles was explored.The data of the two types of models are compared,and the calculation results are analyzed.It is found that the concentration of actin filaments,the arc of actin filament bundles,the concentration of actin filament bundles,and the length of actin filaments in the cell had successively smaller influence on the elastic modulus of the cytoplasmic membrane.When the test conditions were consistent with the simulation conditions,the NST-1400-10 cell stretching system was used to test the changes in cell stiffness caused by changes in the concentration of actin filament-binding bundles.At the cellular and molecular level,the conclusions obtained from the experiments were compared with the results of the simulation analysis.It was found that the stiffness of the plasma membrane of the cell under the external force will increase first and then decrease as the concentration of actin filament bundles increases,which verified the correctness of the model.