Effect Of Palmitoylation Of Caveolin1 On The Structures Of Caveolae

Palmitoylation is one kind of post-translational modification of proteins,and is a reversible protein modification.The occurrence of palmitoylation refers to the addition of palmitoyl group to the cysteine thiol group of a protein,which plays an important role in protein localization,interaction and stability.Palmitoylation is catalyzed by a class of protein termed palmitoyl transferases.Considering that its active center contains a conserved DHHC motif,so it is also called the DHHC family.Caveolin1(CAV1)is an important structure protein of Caveolae.Caveolaes are flask-shaped pits on plasma membrane that paly important roles in material transport and signal transduction.CAV1 can be palmitoylated on Cys133,Cys143 and Cys 156.At present,it is not clear that which palmitoyl transferase palmitoylates CAV1 and the significance of the palmitoylation of CAV1.This is what this article focuses on.In our previous research,palmitoylated proteins were captured by Acyl-RAC assay after isolating of lipid droplet fractions in white adipose tissue and then subjected to Mass Spectrometry analysis.The results showed that the purified protein including CAV1.Real-time qPCR results showed that palmitoyl transferases that were more highly expression in adipose tissue were DHHC2,DHHC4,DHHC5,DHHC6,DHHC7 and DHHC20.Through Acyl-RAC assay analysis,we found that the palmitoyl transferase of CAV1 is DHHC20.And also found that palmitoylation of CAV1 had no effect on the interaction between CAV1 and Cavin1,Plasma Membrane Localization of CAV1 Requires DHHC20,Formation or Stability of Caveolae Requires DHHC20,which has profound significance for studying the significance of CAV1 palmitoylation and the formation or stability of Caveolae.